Evidence map›Paper›PMID 32054086›Full record

ArticleJournal of clinical medicine2020

Pulp-Derived Exosomes in a Fibrin-Based Regenerative Root Filling Material.

Anja Ivica, Chafik Ghayor, Matthias Zehnder, Silvio Valdec, Franz E Weber

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of clinical medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
34citing papers in PubMed, 1 pooled it
2.6field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

34 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.

  1. Pooled it
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  6. Isolation methods of exosomes derived from dental stem cells.International journal of oral science · 2025
    Review
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  8. Article
  9. Review
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  12. Emerging roles of exosomes in oral diseases progression.International journal of oral science · 2024
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Anja IvicaOral Biotechnology and Bioengineering; Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
Chafik GhayorOral Biotechnology and Bioengineering; Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
Matthias ZehnderPreventive Dentistry, Periodontology and Cariology, Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
Silvio ValdecCranio-Maxillofacial and Oral Surgery, Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
Franz E WeberOral Biotechnology and Bioengineering; Center for Dental Medicine, University of Zurich, 8032 Zurich, Switzerland.
University of Zurich · CH

Funding

Bundesstipendium -
6 · The paper itself

Abstract

Regenerative endodontics has been described as a paradigm shift in dentistry, despite its current limitation to immature teeth and reparative rather than regenerative outcomes. Cell-free treatments are favored because of regulatory issues. However, the recruitment of host-derived stem cells to the desired site remains challenging. We investigated whether dental pulp-derived exosomes, which are extracellular vesicles that contain proteins, lipids, RNA, and DNA and thus mirror their parental cells, may be used for this purpose. The use of exosomes may present appreciable advantages over the direct use of transplanted stem cells due to a higher safety profile, easier isolation, preservation, and handling. Here we harvested exosomes from a cultured third-molar pulp cell and assessed them by transmission electron microscopy and Western blotting. Human mesenchymal stem cells (MSCs) were exposed to these exosomes to assess exosome uptake, cell migration, and proliferation. In addition, a fibrin gel (i.e., a diluted fibrin sealant), was assessed as a delivery system for the exosomes. Our results show that exosomes attracted MSCs, and the fibrin gel enhanced their effect. Moreover, exosomes improved the proliferation of MSCs. Therefore, we propose that pulp-derived exosomes in combination with a fibrin gel could be a powerful combination for clinical translation towards improved cell-free regenerative endodontics and thus represent a new way to fill dental hard tissues.

Indexed as

dental pulpexosomesfibrin gelregenerative endodontics

Identifiers

PMID32054086
PMCPMC7074310
OpenAlexW3005652015

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.