ArticlePloS one2020
Proteomic characterization and comparison of ram (Ovis aries) and buck (Capra hircus) spermatozoa proteome using a data independent acquisition mass spectometry (DIA-MS) approach.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 31 citations in OpenAlex.
- Global Proteomic Analysis of Sperm and Seminal Plasma From Tropically-Adapted Rams With Contrasting Parameters of Frozen-Thawed Semen.Molecular reproduction and development · 2026Article
- Identification of metabolic pathways associated with cytoplasmic male sterility in cotton (Gossypium hirsutum L.) using Data-Independent Acquisition-based proteomic profiling.BMC plant biology · 2026Article
- Article
- Sperm capacitation in bulls, rams, bucks, and boars: molecular mechanisms and species-specific differences.Frontiers in veterinary science · 2026Review
- Appraisal of the Use of Proteomics Methodological Approaches and Technologies on Sheep and Goat Research and Clinical Work.Animals : an open access journal from MDPI · 2025Article
- Study on the Polarization of Astrocytes in the Optic Nerve Head of Rats Under High Intraocular Pressure: In Vitro.Bioengineering (Basel, Switzerland) · 2025Article
- Extender development for optimal cryopreservation of buck sperm to increase reproductive efficiency of goats.Frontiers in veterinary science · 2025Review
- Dataset of Panda sperm proteome.Data in brief · 2024Article
- Punicalagin Protects Ram Sperm from Oxidative Stress by Enhancing Antioxidant Capacity and Mitochondrial Potential during Liquid Storage at 4 °C.Animals : an open access journal from MDPI · 2024Article
- Regulatory T cells play a crucial role in maintaining sperm tolerance and male fertility.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- TMT-Based Comparative Proteomic Analysis of the Spermatozoa of Buck (Capra hircus) and Ram (Ovis aries).Genes · 2023Article
- Effect of lactoferrin on ram sperm motility after cryopreservation.Animal bioscience · 2022Article
- Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.Nature cardiovascular research · 2022Article
- Transcriptome-wide mBMC genomics · 2021Article
- Effect of insulin-like growth factor-1 complex of Simmental bull seminal plasma on post-thawed Kacang buck semen fertility.Veterinary world · 2021Article
- Article
- Protein Identification of Spermatozoa and Seminal Plasma in Bottlenose Dolphin (Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fresh semen is most commonly used in an artificial insemination of small ruminants, because of low fertility rates of frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep and goats are classified as one species. In order to optimize sperm cryopreservation protocols in sheep and goat, differences in sperm proteomes between ram and buck are necessary to investigate, which may contribute to differences in function and fertility of spermatozoa. In the current work, a data-independent acquisition-mass spectrometry proteomic approach was used to characterize and make a comparison of ram (Ovis aries) and buck (Capra hircus) sperm proteomes. A total of 2,109 proteins were identified in ram and buck spermatozoa, with 238 differentially abundant proteins. Proteins identified in ram and buck spermatozoa are mainly involved in metabolic pathways for generation of energy and diminishing oxidative stress. Specifically, there are greater abundance of spermatozoa proteins related to the immune protective and capacity activities in ram, while protein that inhibit sperm capacitation shows greater abundance in buck. Our results not only provide novel insights into the characteristics and potential activities of spermatozoa proteins, but also expand the potential direction for sperm cryopreservation in ram and buck.
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