ArticleMicrobial biotechnology2020
Establishment of recombineering genome editing system in Paraburkholderia megapolitana empowers activation of silent biosynthetic gene clusters.
Article in Microbial biotechnology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 31 citations in OpenAlex.
- RECC: A Red/ET-CRISPR/Cas9-based system enabling genome mining of marineSynthetic and systems biotechnology · 2026Article
- Identifying phenotypic and genetic traits for assessing pathogenic potential and biocontrol capacity in Burkholderia sensu lato strains.The ISME journal · 2026Article
- A survey of Burkholderiales secondary metabolites using library-scale stable isotope labeling reveals selective production of methyl-esterified 3-hydroxybutyrate oligomers.Journal of industrial microbiology & biotechnology · 2026Article
- Complete genome sequence and mass spectrometry assist mining siderophore turnerbactin analogs in a non-modelFrontiers in microbiology · 2026Article
- Genome mining, structural elucidation and surface-active property of a new lipopeptide from Bacillus subtilis.Microbial cell factories · 2025Article
- Genomics-Driven Discovery of Plantariitin A, a New Lipopeptide inMolecules (Basel, Switzerland) · 2025Article
- Biosynthesis and engineering of the nonribosomal peptides with a C-terminal putrescine.Nature communications · 2023Article
- Biosynthetic gene cluster synteny: Orthologous polyketide synthases in Hypogymnia physodes, Hypogymnia tubulosa, and Parmelia sulcata.MicrobiologyOpen · 2023Article
- The emerging role of recombineering in microbiology.Engineering microbiology · 2023Review
- Recombineering enables genome mining of novel siderophores in a non-modelEngineering microbiology · 2023Article
- Biosynthetic Potential ofJournal of fungi (Basel, Switzerland) · 2023Article
- Recombineering using RecET-like recombinases from Xenorhabdus and its application in mining of natural products.Applied microbiology and biotechnology · 2022Article
- Recombineering in Non-Model Bacteria.Current protocols · 2022Article
- Biotechnology approaches for natural product discovery, engineering, and production based on Burkholderia bacteria.Current opinion in biotechnology · 2022Review
- Improved dsDNA recombineering enables versatile multiplex genome engineering of kilobase-scale sequences in diverse bacteria.Nucleic acids research · 2022Article
- Rational construction of genome-reduced Burkholderiales chassis facilitates efficient heterologous production of natural products from proteobacteria.Nature communications · 2021Article
- Alkaloids in Contemporary Drug Discovery to Meet Global Disease Needs.Molecules (Basel, Switzerland) · 2021Review
- Genomics-Driven Activation of Silent Biosynthetic Gene Clusters inMolecules (Basel, Switzerland) · 2021Article
- The Hidden Genomic Diversity, Specialized Metabolite Capacity, and Revised Taxonomy ofFrontiers in microbiology · 2021Article
- Mining for novel antibiotics in the age of antimicrobial resistance.Microbial biotechnology · 2020Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Burkholderiales are an emerging source of bioactive natural products. Their genomes contain a large number of cryptic biosynthetic gene clusters (BGCs), indicating great potential for novel structures. However, the lack of genetic tools for the most of Burkholderiales strains restricts the mining of these cryptic BGCs. We previously discovered novel phage recombinases Redαβ7029 from Burkholderiales strain DSM 7029 that could help in efficiently editing several Burkholderiales genomes and established the recombineering genome editing system in Burkholderialse species. Herein, we report the application of this phage recombinase system in another species Paraburkholderia megapolitana DSM 23488, resulting in activation of two silent non-ribosomal peptide synthetase/polyketide synthase BGCs. A novel class of lipopeptide, haereomegapolitanin, was identified through spectroscopic characterization. Haereomegapolitanin A represents an unusual threonine-tagged lipopeptide which is longer than the predicted NRPS assembly line. This recombineering-mediated genome editing system shows great potential for genetic manipulation of more Burkholderiales species to activate silent BGCs for bioactive metabolites discovery.
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