Evidence map›Paper›PMID 32051829›Full record

ArticleBioMed research international2020

MicroRNA-127 Inhibits the Progression of Melanoma by Downregulating Delta-Like Homologue 1.

Ping Tian, Ling Tao, Yujun Wang, Xiaobing Han

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BioMed research international, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 11 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Ping TianXinyang Vocational and Technical College Medical School, Xinyang 464000, China.
Ling TaoXinyang Vocational and Technical College Medical School, Xinyang 464000, China.
Yujun WangDepartment of Gastroenterology, Xinyang Central Hospital, Xinyang 464000, China.
Xiaobing HanDepartment of Oncology, Xinyang Central Hospital, Xinyang 464000, China.ORCID https://orcid.org/0000-0002-7222-8923
Xinyang Central Hospital · CNXinyang College of Agriculture and Forestry · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveMelanoma is the most common form of skin cancer with low survival rate and poor prognosis. MicroRNAs (miRNAs) have been reported to play essential roles in progression of melanoma. However, the role and mechanism of miR-127 in the process of melanoma remain poorly understood.

methodsThe expressions of miR-127 and delta-like homologue 1 (DLK1) were measured in melanoma tissues and cells by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot. Cell proliferation and apoptosis were measured by MTT assay, flow cytometry, and Western blot. The interaction between miR-127 and DLK1 was investigated by bioinformatics analysis, luciferase activity assay, and RNA immunoprecipitation (RIP). Murine xenograft model was conducted to investigate the effect of miR-127 on tumor growth in vivo.

resultsmiR-127 was inhibited and DLK1 mRNA was enhanced in melanoma tissues and cells. Low abundance of miR-127 in melanoma tissues predicted a poor prognosis and was associated with the malignant clinicopathological features. Overexpression of miR-127 inhibited cell proliferation and induced apoptosis in melanoma cells. Moreover, DLK1 was targeted by miR-127 and its restoration reversed the regulatory effect of miR-127 on the process of melanoma. Besides, the addition of miR-127 suppressed xenograft tumor growth via suppressing DLK1 protein level in nude mice.

conclusionmiR-127 blocked the development of melanoma by targeting DLK1, providing a novel biomarker for the treatment of melanoma.

Indexed as

Disease ProgressionAnimalsApoptosisCalcium-Binding ProteinsCell Line, TumorCell ProliferationDown-RegulationFemaleGene Expression Regulation, NeoplasticHumansMaleMelanomaMembrane ProteinsMiceMice, Inbred BALB CMice, NudeCalcium-Binding ProteinsDLK1 protein, humanMembrane ProteinsMicroRNAsMIRN127 microRNA, humanRNA, Messenger

Identifiers

PMID32051829
PMCPMC6995326
OpenAlexW3002650757

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.