Evidence map›Paper›PMID 32051510›Full record

ArticleScientific reports2020

A Targeted Quantitative Proteomic Method Revealed a Substantial Reprogramming of Kinome during Melanoma Metastasis.

Weili Miao, Lin Li, Xiaochuan Liu, Tianyu F Qi, Lei Guo, Ming Huang, Yinsheng Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 8 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Weili MiaoDepartment of Chemistry, University of California, Riverside, CA, 92521-0403, USA.
Lin LiDepartment of Chemistry, University of California, Riverside, CA, 92521-0403, USA.
Xiaochuan LiuDepartment of Chemistry, University of California, Riverside, CA, 92521-0403, USA.
Tianyu F QiEnvironmental Toxicology Graduate Program, University of California, Riverside, CA, 92521-0403, USA.
Lei GuoEnvironmental Toxicology Graduate Program, University of California, Riverside, CA, 92521-0403, USA.
Ming HuangEnvironmental Toxicology Graduate Program, University of California, Riverside, CA, 92521-0403, USA.
Yinsheng WangDepartment of Chemistry, University of California, Riverside, CA, 92521-0403, USA. yinsheng.wang@ucr.edu.ORCID http://orcid.org/0000-0001-5565-283X
University of California, Riverside · US

Funding

Research Training in Environmental ToxicologyT32ES018827 · NIEHS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI Yinsheng Wang · 2010 to 2026
$5.3M
Quantitative Adductomics Approaches for Assessing the Occurrence and Repair of DNA AdductsR01CA210072 · NCI · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI WANG, YINSHENG · 2017 to 2021
$1.8M
NCI NIH HHS R01 CA210072NIEHS NIH HHS T32 ES018827
6 · The paper itself

Abstract

Kinases are involved in numerous critical cell signaling processes, and dysregulation in kinase signaling is implicated in many types of human cancers. In this study, we applied a parallel-reaction monitoring (PRM)-based targeted proteomic method to assess kinome reprogramming during melanoma metastasis in three pairs of matched primary/metastatic human melanoma cell lines. Around 300 kinases were detected in each pair of cell lines, and the results showed that Janus kinase 3 (JAK3) was with reduced expression in the metastatic lines of all three pairs of melanoma cells. Interrogation of The Cancer Genome Atlas (TCGA) data showed that reduced expression of JAK3 is correlated with poorer prognosis in melanoma patients. Additionally, metastatic human melanoma cells/tissues exhibited diminished levels of JAK3 mRNA relative to primary melanoma cells/tissues. Moreover, JAK3 suppresses the migration and invasion of cultured melanoma cells by modulating the activities of matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9). In summary, our targeted kinome profiling method provided by far the most comprehensive dataset for kinome reprogramming associated with melanoma progression, which builds a solid foundation for examining the functions of other kinases in melanoma metastasis. Moreover, our results reveal a role of JAK3 as a potential suppressor for melanoma metastasis.

Indexed as

Gene Expression Regulation, NeoplasticCell Line, TumorHumansJanus Kinase 3Matrix MetalloproteinasesMelanomaNeoplasm MetastasisProteomeJAK3 protein, humanJanus Kinase 3Matrix MetalloproteinasesProteome

Identifiers

PMID32051510
PMCPMC7015909
OpenAlexW3005858453

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.