ArticleRevista do Instituto de Medicina Tropical de Sao Paulo2020
A Real Time PCR strategy for the detection and quantification of Candida albicans in human blood.
Article in Revista do Instituto de Medicina Tropical de Sao Paulo, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 14 citations in OpenAlex.
- Detection of Candida albicans by a sandwich assay using boronate affinity microspheres coupled with amphotericin B-time-resolved fluorescent microspheres.Analytical and bioanalytical chemistry · 2026Article
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- Rapid and Sensitive Detection ofBiosensors · 2026Article
- The Role of the OLM CandID Real-Time PCR in the Invasive Candidiasis Diagnostic Surveillance in Intensive Care Unit Patients.Microorganisms · 2025Article
- Metagenomic Next-Generation Sequencing Unveils Prognostic Microbial Synergism and Guides Precision Therapy in Candidemia: A Retrospective Cohort Study.Infection and drug resistance · 2025Article
- Robust Fluorometric Aptamer Assay for Direct and Rapid Detection of Clinical Isolates ofInternational journal of molecular sciences · 2024Article
- Innovative Biosensing Approaches for Swift Identification ofLife (Basel, Switzerland) · 2023Review
- Biosensors and Diagnostics for Fungal Detection.Journal of fungi (Basel, Switzerland) · 2020Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candidemia is a significant cause of bloodstream infections (BSI) in nosocomial settings. The identification of species can potentially improve the quality of care and decrease human mortality. Quantitative PCR (qPCR) was evaluated for Candida albicans detection using culture suspensions containing C. albicans , spiked human blood, the cloned qPCR target fragment (ITS2 region) and the results of these assays were compared. The assays showed a good detection limit: C. albicans DNA extracted from yeast (sensitivity 0.2 CFU/µL), spiked human blood (sensitivity 10 CFU/mL), and cloned fragment of ITS2 region (sensitivity 20 target copies/μL). The efficiency of ITS2 fragment-qPCR ranged from 89.67 to 97.07, and the linearity (R2) of the standard curve ranged from 0.992 to 0.999. The results showed that this ITS2-qPCR has a great potential as a molecular prototype model for the development of a test to be applied in clinical practice, greatly reducing the time of candidemia diagnosis, which is extremely important in this clinical setting.
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