ArticleDevelopmental cell2020
Targeted De Novo Centromere Formation in Drosophila Reveals Plasticity and Maintenance Potential of CENP-A Chromatin.
Article in Developmental cell, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Epigenetic landscape of synthetic human centromere core regions: CENP-A assembly and euchromatic modifications interdependently antagonize heterochromatin accumulation.Nucleic acids research · 2026Article
- Dual pathways via CENP-C and Mis18C recruit HJURP for CENP-A deposition into vertebrate centromeres.The EMBO journal · 2026Article
- The functional organization of chromosome territories in single nuclei during zygotic genome activation.Scientific reports · 2026Article
- Microbial interactions of EDTA: recent advances and biological applications in the context of natural product modulation.Frontiers in antibiotics · 2026Review
- Increased maize chromosome number by engineered chromosome fission.Science advances · 2025Article
- Transcription of a centromere-enriched retroelement and local retention of its RNA are significant features of the CENP-A chromatin landscape.Genome biology · 2024Article
- Turnover of retroelements and satellite DNA drives centromere reorganization over short evolutionary timescales in Drosophila.PLoS biology · 2024Article
- The Chaperone NASP Contributes to de Novo Deposition of the Centromeric Histone Variant CENH3 in Arabidopsis Early Embryogenesis.Plant & cell physiology · 2024Article
- Article
- Higher-order protein assembly controls kinetochore formation.Nature cell biology · 2024Article
- Centromere structure and function: lessons from Drosophila.Genetics · 2023Article
- Centromere-Proximal Suppression of Meiotic Crossovers inbioRxiv : the preprint server for biology · 2023Article
- Centromere Plasticity With Evolutionary Conservation and Divergence Uncovered by Wheat 10+ Genomes.Molecular biology and evolution · 2023Article
- Synthetic maize centromeres transmit chromosomes across generations.Nature plants · 2023Article
- Review
- Incorporation of CENP-A/CID into centromeres during early Drosophila embryogenesis does not require RNA polymerase II-mediated transcription.Chromosoma · 2022Article
- Diverse mechanisms of centromere specification.Current biology : CB · 2021Review
- Induction of spontaneous human neocentromere formation and long-term maturation.The Journal of cell biology · 2021Article
- Biochemical evidence for diverse strategies in the inner kinetochore.Open biology · 2020Review
- Review
Corrections and comments
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- Erratum issued
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Centromeres are essential for accurate chromosome segregation and are marked by centromere protein A (CENP-A) nucleosomes. Mis-targeted CENP-A chromatin has been shown to seed centromeres at non-centromeric DNA. However, the requirements for such de novo centromere formation and transmission in vivo remain unknown. Here, we employ Drosophila melanogaster and the LacI/lacO system to investigate the ability of targeted de novo centromeres to assemble and be inherited through development. De novo centromeres form efficiently at six distinct genomic locations, which include actively transcribed chromatin and heterochromatin, and cause widespread chromosomal instability. During tethering, de novo centromeres sometimes prevail, causing the loss of the endogenous centromere via DNA breaks and HP1-dependent epigenetic inactivation. Transient induction of de novo centromeres and chromosome healing in early embryogenesis show that, once established, these centromeres can be maintained through development. Our results underpin the ability of CENP-A chromatin to establish and sustain mitotic centromere function in Drosophila.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.