ArticlePhotochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology2020
Optogenetic Rac1 engineered from membrane lipid-binding RGS-LOV for inducible lamellipodia formation.
Article in Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Article
- Cytoskeletal disassembly by optogenetic control of RhoA signaling termination.bioRxiv : the preprint server for biology · 2026Article
- A temperature-inducible protein module for control of mammalian cell fate.Nature methods · 2025Article
- A temperature-inducible protein module for control of mammalian cell fate.bioRxiv : the preprint server for biology · 2024Article
- Condensed Matter Systems Exposed to Radiation: Multiscale Theory, Simulations, and Experiment.Chemical reviews · 2024Review
- Opticool: Cutting-edge transgenic optical tools.PLoS genetics · 2024Review
- Optogenetic clustering and membrane translocation of the BcLOV4 photoreceptor.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Development and Application of an Optogenetic Manipulation System to Suppress Actomyosin Activity inInternational journal of molecular sciences · 2023Article
- The expanding role of split protein complementation in opsin-free optogenetics.Current opinion in pharmacology · 2022Review
- Computational framework for single-cell spatiotemporal dynamics of optogenetic membrane recruitment.Cell reports methods · 2022Article
- Temperature-responsive optogenetic probes of cell signaling.Nature chemical biology · 2022Article
- Optogenetics in the hot seat.Nature chemical biology · 2022Article
- Designing Single-Component Optogenetic Membrane Recruitment Systems: The Rho-Family GTPase Signaling Toolbox.ACS synthetic biology · 2022Article
- Resveratrol suppresses malignant progression of oral squamous cell carcinoma cells by inducing the ZNF750/RAC1 signaling pathway.Bioengineered · 2021Article
- Single-Component Optogenetic Tools for Inducible RhoA GTPase Signaling.Advanced biology · 2021Article
- An optogenetic model reveals cell shape regulation through FAK and fascin.Journal of cell science · 2021Article
- Steering Molecular Activity with Optogenetics: Recent Advances and Perspectives.Advanced biology · 2021Review
- A light life together: photosensing in the plant microbiota.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2021Review
- Reverse and Forward Engineering Multicellular Structures with Optogenetics.Current opinion in biomedical engineering · 2020Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We report the construction of a single-component optogenetic Rac1 (opto-Rac1) to control actin polymerization by dynamic membrane recruitment. Opto-Rac1 is a fusion of wildtype human Rac1 small GTPase to the C-terminal region of BcLOV4, a LOV (light-oxygen-voltage) photoreceptor that rapidly binds the plasma membrane upon blue-light activation via a direct electrostatic interaction with anionic membrane phospholipids. Translocation of the fused wildtype Rac1 effector permits its activation by GEFs (guanine nucleotide exchange factors) and consequent actin polymerization and lamellipodia formation, unlike in existing single-chain systems that operate by allosteric photo-switching of constitutively active Rac1 or the heterodimerization-based (i.e. two-component) membrane recruitment of a Rac1-activating GEF. Opto-Rac1 induction of lamellipodia formation was spatially restricted to the patterned illumination field and was efficient, requiring sparse stimulation duty ratios of ∼1-2% (at the sensitivity threshold for flavin photocycling) to cause significant changes in cell morphology. This work exemplifies how the discovery of LOV proteins of distinct signal transmission modes can beget new classes of optogenetic tools for controlling cellular function.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.