ArticlePlant molecular biology2020
Precise evaluation of tissue culture-induced variation during optimisation of in vitro regeneration regime in barley.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- CuCells · 2025Article
- β-glucans, SAM, and GSH fluctuations in barley anther tissue culture conditions affect regenerants' DNA methylation and GPRE.BMC plant biology · 2024Article
- An insight into tissue culture-induced variation origin shared between anther culture-derived triticale regenerants.BMC plant biology · 2024Article
- Effect of copper and silver ions on sequence and DNA methylation changes in triticale regenerants gained via somatic embryogenesis.Journal of applied genetics · 2022Article
- Triticale doubled haploid plant regeneration factors linked by structural equation modeling.Journal of applied genetics · 2022Article
- Article
- Glutathione and copper ions as critical factors of green plant regeneration efficiency of triticaleFrontiers in plant science · 2022Article
- Article
- Article
- Understanding In Vitro Tissue Culture-Induced Variation Phenomenon in Microspore System.International journal of molecular sciences · 2021Review
- Androgenic-Induced Transposable Elements Dependent Sequence Variation in Barley.International journal of molecular sciences · 2021Article
- Barley somatic embryogenesis-an attempt to modify variation induced in tissue culture.Journal of biological research (Thessalonike, Greece) · 2021Article
- The Effect of Zinc, Copper, and Silver Ions on Oat (Plants (Basel, Switzerland) · 2021Article
- Time of In Vitro Anther Culture May Moderate Action of Copper and Silver Ions that Affect the Relationship between DNA Methylation Change and the Yield of Barley Green Regenerants.Plants (Basel, Switzerland) · 2020Article
- Exploring the Biochemical Origin of DNA Sequence Variation in Barley Plants Regenerated via in Vitro Anther Culture.International journal of molecular sciences · 2020Article
- CG Demethylation Leads to Sequence Mutations in an Anther Culture of Barley Due to the Presence of Cu, Ag Ions in the Medium and Culture Time.International journal of molecular sciences · 2020Article
- Copper Ions Induce DNA Sequence Variation in Zygotic Embryo Culture-Derived Barley Regenerants.Frontiers in plant science · 2020Article
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2 authors.
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Abstract
key messageThe Taguchi method and metAFLP analysis were used to optimise barley regenerants towards maximum and minimum levels of tissue culture-induced variation. The subtle effects of symmetric and asymmetric methylation changes in regenerants were identified. Plant tissue cultures (PTCs) provide researchers with unique materials that accelerate the development of new breeding cultivars and facilitate studies on off-type regenerants. The emerging variability of regenerants derived from PTCs may have both genetic and epigenetic origins, and may be desirable or degrade the value of regenerated plants. Thus, it is crucial to determine how the PTC variation level can be controlled. The easiest way to manipulate total tissue culture-induced variation (TTCIV) is to utilise appropriate stress factors and suitable medium components. This study describes the optimisation of in vitro tissue culture-induced variation in plant regenerants derived from barley anther culture, and maximizes and minimizes regenerant variation compared with the source explants. The approach relied on methylation amplified fragment length polymorphism (metAFLP)-derived TTCIV characteristics, which were evaluated in regenerants derived under distinct tissue culture conditions and analysed via Taguchi statistics. The factors that may trigger TTCIV included CuSO
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