ArticleScientific reports2020
Power of Scanning Electron Microscopy and Energy Dispersive X-Ray Analysis in Rapid Microbial Detection and Identification at the Single Cell Level.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed.
- Impact of ionic imbalances on rat cardiomyocyte function in pulmonary arterial hypertension: insights from energy dispersive X-ray spectroscopy and scanning electron microscopy analysis.European journal of applied physiology · 2026Article
- From Membrane Composition to Antimicrobial Strategies: Experimental and Computational Approaches to AMP Design and Selectivity.Small (Weinheim an der Bergstrasse, Germany) · 2026Review
- Flexible Copper-Based TEM Grid for Microscopic Characterization of Aged Magnetotactic Bacteria MS-1 and Their Magnetosome Crystals in Air-Dried Droplet.Molecules (Basel, Switzerland) · 2026Article
- From fibres to field: biohybrid coating of soybean (Glycine max. L) seeds with PGPB Pseudomonas urmiensis AW4 or Bacillus pumilus SF5 by electrospinning technology.World journal of microbiology & biotechnology · 2025Article
- Novel Biocompatible Hyaluronic Acid (HA)/Polyvinylpyrrolidone (PVP) Composites Containing Silver Decorated-Zinc MOF Nanoparticles: Antimicrobial Activity, Drug Delivery and Wound Healing.AAPS PharmSciTech · 2025Article
- Influence of Ionic Surfactants on Microfiltration of Enveloped and Nonenveloped Viruses.Water environment research : a research publication of the Water Environment Federation · 2025Article
- Article
- Effects of Detoxifying Substances on Uranium Removal by Bacteria Isolated from Mine Soils: Performance, Mechanisms, and Bacterial Communities.Microbial ecology · 2024Article
- Regulation of symbiotic interactions and primitive lichen differentiation by UMP1 MAP kinase in Umbilicaria muhlenbergii.Nature communications · 2023Article
- Article
- Remineralization of early enamel lesions with a novel prepared tricalcium silicate paste.Scientific reports · 2022Article
- Preparation of Smart Surfaces Based on PNaSS@PEDOT Microspheres: Testing ofSensors (Basel, Switzerland) · 2022Article
- A preliminary investigation into bacterial viability using scanning electron microscopy-energy-dispersive X-ray analysis: The case of antibiotics.Frontiers in microbiology · 2022Article
- Resonantly Enhanced Difference-Frequency Generation in the Core X-ray Absorption of Molecules.The journal of physical chemistry. A · 2021Article
- Advancements in Assessments of Bio-Tissue Engineering and Viable Cell Delivery Matrices Using Bile Acid-Based Pharmacological Biotechnologies.Nanomaterials (Basel, Switzerland) · 2021Review
- Antimicrobial TiOChemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Fossil microbial shark tooth decay documents in situ metabolism of enameloid proteins as nutrition source in deep water environments.Scientific reports · 2020Article
- Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The demand for rapid, consistent and easy-to-use techniques for detecting and identifying pathogens in various areas, such as clinical diagnosis, the pharmaceutical industry, environmental science and food inspection, is very important. In this study, the reference strains of six food-borne pathogens, namely, Escherichia coli 0157: H7 ATCC 43890, Cronobacter sakazakii ATCC 29004, Salmonella Typhimurium ATCC 43971, Staphylococcus aureus KCCM 40050, Bacillus subtilis ATCC 14579, and Listeria monocytogenes ATCC 19115, were chosen for scanning electron microscopy (SEM) and energy dispersive X-ray (EDX) analysis. In our study, the time-consuming sample preparation step for the microbial analysis under SEM was avoided, which makes this detection process notably rapid. Samples were loaded onto a 0.01-µm-thick silver (Ag) foil surface to avoid any charging effect. Two different excitation voltages, 10 kV and 5 kV, were used to determine the elemental information. Information obtained from SEM-EDX can distinguish individual single cells and detect viable and nonviable microorganisms. This work demonstrates that the combination of morphological and elemental information obtained from SEM-EDX analysis with the help of principal component analysis (PCA) enables the rapid identification of single microbial cells without following time-consuming microbiological cultivation methods.
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