ArticleFluids and barriers of the CNS2020
Choroid plexus LAT2 and SNAT3 as partners in CSF amino acid homeostasis maintenance.
Article in Fluids and barriers of the CNS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 20 citations in OpenAlex.
- Glass scallop genome reveals key adaptations to deep-sea environments and ectosymbiosis.Nature communications · 2026Article
- Age-related molecular and functional remodeling of the choroid plexus in the gray mouse lemur.PloS one · 2026Article
- GABAergic MicroRNA-34a in the Dorsal Raphe Nuclei Contributes to Circulating MicroRNA-34a Levels.Molecular neurobiology · 2025Article
- Apolipoprotein-L1 G1 variant contributes to hydrocephalus but not to atherosclerosis in apolipoprotein-E knock-out mice.Clinical science (London, England : 1979) · 2025Article
- Defining diurnal fluctuations in mouse choroid plexus and CSF at high molecular, spatial, and temporal resolution.Nature communications · 2023Article
- Localized proteomic differences in the choroid plexus of Alzheimer's disease and epilepsy patients.Frontiers in neurology · 2023Article
- A Survey of the Metabolic Landscape of the Developing Cerebellum at Single-Cell Resolution.Cerebellum (London, England) · 2022Article
- Targeting choroid plexus epithelium as a novel therapeutic strategy for hydrocephalus.Journal of neuroinflammation · 2022Review
- Transcriptional profiling of transport mechanisms and regulatory pathways in rat choroid plexus.Fluids and barriers of the CNS · 2022Article
- Biallelic variants in SLC38A3 encoding a glutamine transporter cause epileptic encephalopathy.Brain : a journal of neurology · 2022Article
- Heteromeric Amino Acid Transporters in Brain: from Physiology to Pathology.Neurochemical research · 2022Review
- Establishing a competing endogenous RNA (ceRNA)-immunoregulatory network associated with the progression of Alzheimer's disease.Annals of translational medicine · 2022Article
- Dysregulation of Astrocytic Glutamine Transport in Acute Hyperammonemic Brain Edema.Frontiers in neuroscience · 2022Review
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Authors and funding
7 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundCerebrospinal fluid (CSF) is mainly produced by the choroid plexus (CP) located in brain ventricles. Although derived from blood plasma, it is nearly protein-free (~ 250-fold less) and contains about 2-20-fold less free amino acids, with the exception of glutamine (Gln) which is nearly equal. The aim of this study was to determine which amino acid transporters are expressed in mouse CP epithelium in order to gain understanding about how this barrier maintains the observed amino acid concentration gradient.
methodsExpression of amino acid transporters was assessed in isolated choroid plexuses (CPs) by qRT-PCR followed by localization studies using immunofluorescence with specific antibodies. The impact of LAT2 (Slc7a8) antiporter deletion on CSF amino acids was determined.
resultsThe purity of isolated choroid plexuses was tested on the mRNA level using specific markers, in particular transthyretin (Ttr) that was enriched 330-fold in CP compared to cerebral tissue. In a first experimental round, 14 out of 32 Slc amino acid transporters tested on the mRNA level by qPCR were selected for further investigation. Out of these, five were considered highly expressed, SNAT1 (Slc38a1), SNAT3 (Slc38a3), LAT2 (Slc7a8), ASC1 (Slc7a10) and SIT1 (Slc6a20b). Three of them were visualized by immunofluorescence: SNAT1 (Slc38a1), a neutral amino acid-Na
conclusionThese results suggest that Gln is actively transported by SNAT1 from the blood into CP epithelial cells and then released luminally into CSF via SNAT3 and LAT2. Its efflux via LAT2 may drive the reuptake from the CSF of essential amino acid substrates of this antiporter and thereby participates to maintaining the amino acid gradient between plasma and CSF.
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