Evidence map›Paper›PMID 32046751›Full record

ArticleJournal of ovarian research2020

Integrative analysis of transcription factors and microRNAs in ovarian cancer cell spheroids.

Hyun Park, Sohyun Hwang, Ju-Yeon Jeong, Sang Geun Jung, Min Chul Choi, Won Duk Joo, Seung Hun Song, Chan Lee, Hee Jung An

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of ovarian research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 37% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Review
  3. Cancers · 2021
    Article
  4. Article
  5. Review
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Hyun ParkDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Sohyun HwangDepartment of Pathology, College of Medicine, CHA University, Seongnam, 351 Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 13496, South Korea.
Ju-Yeon JeongDepartment of Pathology, College of Medicine, CHA University, Seongnam, 351 Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 13496, South Korea.
Sang Geun JungDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Min Chul ChoiDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Won Duk JooDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Seung Hun SongDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Chan LeeDivision of Gynecologic Oncology, Department of Obstetrics and Gynecology, College of Medicine, CHA University, Seongnam, Gyeonggi-do, South Korea.
Hee Jung AnDepartment of Pathology, College of Medicine, CHA University, Seongnam, 351 Yatap-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, 13496, South Korea. hjahn@cha.ac.kr.
CHA University · KR

Funding

Korea Health Industry Development Institute HI16C1559Ministry of Education 2017R1D1A1B03035627
6 · The paper itself

Abstract

backgroundCancer stem cells (CSCs) can self-renew, proliferate into differentiated cells, or enter a quiescent state and are regarded to cause chemoresistance and recurrence. An integrative analysis of transcription factors (TF) and miRNAs was performed in ovarian CSC-enriched spheroid-forming cells (SFCs) to identify factors relevant to ovarian CSCs.

methodsFresh tumor cells from three ovarian cancer patients were cultured in standard and in selective medium. The mRNAs and miRNAs that exhibited significant differential expression between SFCs and adherent cells were identified using mRNA and miRNAs microarrays. Target genes of miRNAs were further selected if predicted with TargetScan by half of the miRNAs or more. Gene enrichment analysis was performed on over- or under-expressed mRNAs and target genes of miRNAs using DAVID tools. Complex regulatory networks were combined from TF-genes and miRNA-genes interactions using the MAGIA webtool.

resultsA total of 1245 mRNA and 55 miRNAs were differentially expressed (p-value< 0.05, paired t-test). Elevation of transcription-related processes and suppression of focal adhesion pathway were noted in SFCs, according to the enrichment analyses. Transcriptional hyperactivity is a known characteristic of the stem cell transcriptome. The integrative network suggested that cell cycle was arrested in SFCs where over-expressed EGR1 and under-expressed MYC and miR-130a-3p had multiple connections with target genes.

conclusionsMYC, EGR1, and miR-130a-3p were hubs in our integrative analysis of ovarian CSC-enriched SFCs, suggesting that ovarian cancer SFCs display a stem cell identity with the quiescent phenotype where adhesion- and cell cycle-related genes were suppressed.

Indexed as

FemaleHumansMicroRNAsOvarian NeoplasmsSpheroids, CellularTranscription FactorsMicroRNAsTranscription FactorsCancer stem cellsmicroRNAsOvarian epithelial carcinomaSpheroid-forming cellsTranscription factors

Identifiers

PMID32046751
PMCPMC7014770
OpenAlexW3006790259

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.