Observational studyReproductive sciences (Thousand Oaks, Calif.)2020
The Vaginal Microbiome as a Tool to Predict rASRM Stage of Disease in Endometriosis: a Pilot Study.
Observational study in Reproductive sciences (Thousand Oaks, Calif.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 5 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
54 citing papers in PubMed, 5 syntheses or guidelines pooled it, 91 citations in OpenAlex.
- Gut Microbiota Alterations and Reproductive Tract Dysbiosis in Endometriosis: A Systematic Review.Medicina (Kaunas, Lithuania) · 2026Pooled it
- Impact of hormonal treatments for endometriosis on the reproductive microbiome: a systematic review.Frontiers in microbiology · 2026Pooled it
- Meta-Analysis of 16S rRNA Sequencing Reveals Altered Fecal but Not Vaginal Microbial Composition and Function in Women with Endometriosis.Medicina (Kaunas, Lithuania) · 2025Pooled it
- Correlation between dysbiosis of vaginal microecology and endometriosis: A systematic review and meta-analysis.PloS one · 2024Pooled it
- Gut and Vaginal Microbiota in the Endometriosis: Systematic Review and Meta-Analysis.BioMed research international · 2023Pooled it
- The Female Reproductive Microbiome: Mechanistic Insights and Bioengineering Perspectives.Biology · 2026Review
- Suggestive associations between genetically predicted gut microbiota and endometriosis: a two-sample Mendelian randomization study.Journal of medical microbiology · 2026Article
- Molecular Insights Into Endometriosis for Early Detection and Therapeutic Targets.Reproductive sciences (Thousand Oaks, Calif.) · 2026Review
- Translational Assessment of Omics Approaches in Endometriosis: Bridging Molecular Discovery with Clinical Utility.International journal of molecular sciences · 2026Review
- Citizen science reveals comorbidities in endometriosis with no shift in vaginal microbiome composition.npj women's health · 2026Article
- Decoding the reproductive microbiome: enabling clinical and biological insights through machine and deep learning.Frontiers in endocrinology · 2026Review
- Microbiota insights in endometriosis.Microbiome · 2025Review
- The Role of the Microbiome in Endometriosis.Reproductive sciences (Thousand Oaks, Calif.) · 2025Review
- Evaluation of inflammatory serum parameters as a diagnostic tool in patients with endometriosis: a case-control study.Scientific reports · 2025Article
- The Multifactorial Pathogenesis of Endometriosis: A Narrative Review Integrating Hormonal, Immune, and Microbiome Aspects.Medicina (Kaunas, Lithuania) · 2025Review
- Gut microbiome in patients with early-stage and late-stage endometriosis.BMC women's health · 2025Article
- Gut-X axis.iMeta · 2025Review
- Oral, Vaginal, and Stool Microbial Signatures in Patients With Endometriosis as Potential Diagnostic Non-Invasive Biomarkers: A Prospective Cohort Study.BJOG : an international journal of obstetrics and gynaecology · 2025Article
- The sow vaginal and gut microbiota associated with longevity and reproductive performance.Journal of animal science and biotechnology · 2025Article
- Reproductive tract microbiome dysbiosis associated with gynecological diseases.Frontiers in cellular and infection microbiology · 2025Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
Endometriosis remains a challenge to understand and to diagnose. This is an observational cross-sectional pilot study to characterize the gut and vaginal microbiome profiles among endometriosis patients and control subjects without the disease and to explore their potential use as a less-invasive diagnostic tool for endometriosis. Overall, 59 women were included, n = 35 with endometriosis and n = 24 controls. Rectal and vaginal samples were collected in two different periods of the menstrual cycle from all subjects. Gut and vaginal microbiomes from patients with different rASRM (revised American Society for Reproductive Medicine) endometriosis stages and controls were analyzed. Illumina sequencing libraries were constructed using a two-step 16S rRNA gene PCR amplicon approach. Correlations of 16S rRNA gene amplicon data with clinical metadata were conducted using a random forest-based machine-learning classification analysis. Distribution of vaginal CSTs (community state types) significantly differed between follicular and menstrual phases of the menstrual cycle (p = 0.021, Fisher's exact test). Vaginal and rectal microbiome profiles and their association to severity of endometriosis (according to rASRM stages) were evaluated. Classification models built with machine-learning methods on the microbiota composition during follicular and menstrual phases of the cycle were built, and it was possible to accurately predict rASRM stages 1-2 verses rASRM stages 3-4 endometriosis. The feature contributing the most to this prediction was an OTU (operational taxonomic unit) from the genus Anaerococcus. Gut and vaginal microbiomes of women with endometriosis have been investigated. Our findings suggest for the first time that vaginal microbiome may predict stage of disease when endometriosis is present.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.