ArticleMolecular therapy. Nucleic acids2020
The Circular RNA circHUWE1 Sponges the miR-29b-AKT3 Axis to Regulate Myoblast Development.
Article in Molecular therapy. Nucleic acids, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 80 citations in OpenAlex.
- circMAC: microRNA-conditioned binding-site localization on circular RNA isoforms.Briefings in bioinformatics · 2026Article
- METTL3-mediated N6-methyladenosine modification of circHUWE1 promotes high glucose-induced cardiomyocyte apoptosis and ferroptosis-related alterations through regulating miR-671-5p/CELF1 axis.Molecular and cellular biochemistry · 2026Article
- CircRNACells · 2026Article
- Screening and Functional Transformation Analysis of Genes Related to Skeletal Muscle Development in Supplemental-Fed Oula Sheep.Animals : an open access journal from MDPI · 2025Article
- Exploring the landscape of circular RNAs in the development of skeletal muscle in Huaxi cattle.BMC genomics · 2025Article
- Circ_HUWE1: a novel regulator of lipid accumulation, inflammation, and gut microbiota in atherosclerosis.Cell & bioscience · 2025Article
- A novel circular RNA circ_0020647 promotes ETEC-induced IPEC-J2 cell pyroptosis via the ssc-miR-185/BRD4 axis.Frontiers in veterinary science · 2025Article
- Circular RNAs and their emerging roles in muscular immune-related diseases.Frontiers in immunology · 2025Review
- CircRNA profiling of skeletal muscle satellite cells in goats reveals circTGFβ2 promotes myoblast differentiation.BMC genomics · 2024Article
- Role and Regulatory Mechanism of circRNA_14820 in the Proliferation and Differentiation of Goat Skeletal Muscle Satellite Cells.International journal of molecular sciences · 2024Article
- Circ_ST6GAL1-mediated competing endogenous RNA network regulates TGF-β1-stimulated matrix Metalloproteinase-13 expression via Runx2 acetylation in osteoblasts.Non-coding RNA research · 2024Article
- Emerging roles of circular RNAs in stem cells.Genes & diseases · 2023Review
- CircTmeff1 Promotes Muscle Atrophy by Interacting with TDP-43 and Encoding A Novel TMEFF1-339aa Protein.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- The novel circular RNA CircMef2c is positively associated with muscle growth in Nile tilapia.Genomics · 2023Article
- Biological functions of circRNAs and their advance on skeletal muscle development in bovine.3 Biotech · 2023Review
- Article
- Recent Progress on Circular RNAs in the Development of Skeletal Muscle and Adipose Tissues of Farm Animals.Biomolecules · 2023Review
- Review
- Circular RNA regulation of fat deposition and muscle development in cattle.Veterinary medicine and science · 2022Review
- Targeting circRNA-MAP4K2 for the treatment of diabetes-induced retinal vascular dysfunction.Aging · 2022Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Myogenesis is controlled by a well-established transcriptional hierarchy that coordinates the activities of a set of muscle genes. Recently, roles in myogenesis have been described for non-coding RNAs, including a role of circular RNA (circRNA) to regulate muscle gene expression. However, the functions of circRNA and the underlying mechanism by which circRNAs affect myogenesis remain poorly understood. In this study, we analyzed circRNA high-throughput sequencing results of bovine skeletal muscle samples and constructed a circRNA-miRNA-mRNA network according to the competitive endogenous RNA (ceRNA) theory. The putative circHUWE1-miR-29b-AKT3 network was analyzed and its involvement in myogenesis was confirmed through a series of assays. To assess the potential function of this regulation, bovine myoblasts were infected with overexpression plasmids and small interfering RNAs (siRNAs) that target circHUWE1. Next, cell proliferation, apoptosis, and differentiation were analyzed using Cell Counting Kit-8 (CCK-8), 5-ethynyl-2'-deoxyuridine (EdU), flow cytometry, western blotting, and qRT-PCR assays. The results suggest that circHUWE1 facilitates bovine myoblast proliferation and inhibits cell apoptosis and differentiation. Next, bioinformatics, dual-luciferase reporter assay, and AGO2 RNA immunoprecipitation (RIP) approaches were used to verify the interaction between circHUWE1, miR-29b, and AKT3. Subsequently, we identified that circHUWE1 could directly interfere with the ability of miR-29b to relieve AKT3 suppression, which ultimately activates the AKT signaling pathway. These findings suggested a new regulatory pathway for bovine skeletal muscle development, and they also expand our understanding of circRNA functions in mammals.
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