ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2020
Effect of Different Proteinase K Digest Protocols and Deparaffinization Methods on Yield and Integrity of DNA Extracted From Formalin-fixed, Paraffin-embedded Tissue.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
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- Nickel Bioaccumulation in Oral Biofilm, Gingival Tissue, and Saliva During Fixed Orthodontic Treatment: A 12-Month Prospective Cohort Study on Predictors and Salivary pH Correlation.Journal of clinical and experimental dentistry · 2025Article
- Association of Mitochondrial DNA Copy Number Variations with Triple-Negative Breast Cancer: A Potential Biomarker Study.Diseases (Basel, Switzerland) · 2025Article
- Population genetic structure of Uroteuthis edulis in the East China Sea based on the COI gene.Molecular biology reports · 2025Article
- Application of DNA Methylome Analysis to Patients with ME/CFS.Methods in molecular biology (Clifton, N.J.) · 2025Article
- A Modified Method for DNA Extraction From Buccal Cells for Genetic Epidemiological Studies Among Children.Cureus · 2024Article
- Improving Yields in Multi-analyte Extractions by Utilizing Post-homogenized Tissue Debris.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2023Article
- New protocol for kinetic assay seeding ability recovery "KASAR" from formalin-fixed paraffin-embedded tissues.Frontiers in molecular biosciences · 2023Article
- Why Formalin-fixed, Paraffin-embedded Biospecimens Must Be Used in Genomic Medicine: An Evidence-based Review and Conclusion.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2020Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA extracted from formalin-fixed, paraffin-embedded tissue sections is often inadequate for sequencing, due to poor yield or degradation. We optimized the proteinase K digest by testing increased volume of enzyme and increased digest length from the manufacturer's protocol using 54 biospecimens, performing the digest in centrifuge tubes. Doubling the quantity of proteinase K resulted in a median increase in yield of 96%. Applying the optimized proteinase K protocol to sections deparaffinized on microscope slides generated a further increase in yield of 41%, but only at >50,000 epithelial tumor cells/section. DNA yield now correlated with (χ
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Registered trials
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