Evidence map›Paper›PMID 32043204›Full record

ArticleJournal of molecular neuroscience : MN2020

TGFβ and Wnt Signaling Pathways Cooperatively Enhance Early Dopaminergic Differentiation of the Unrestricted Somatic Stem Cells.

Azimeh Akhlaghpour, Azita Parvaneh Tafreshi, Eleni Roussa, Claude Bernard, Bahman Zeynali

Abstract read
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In one paragraph

Article in Journal of molecular neuroscience : MN, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.1field-weighted citation impact, top 62% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 4 institutions in 3 countries.

Azimeh AkhlaghpourDevelopmental Biology Lab, School of Biology, College of Science, University of Tehran, Tehran, Iran.
Azita Parvaneh TafreshiDepartment of Molecular Medicine, Faculty of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, 14965-161, Iran. tafreshi@nigeb.ac.ir.ORCID http://orcid.org/0000-0003-1678-2863
Eleni RoussaInstitute of Anatomy and Cell Biology, University of Freiburg, Freiburg, Germany.
Claude BernardAustralian Regenerative Medicine Institute, Monash University, Melbourne, Victoria, Australia.
Bahman ZeynaliDevelopmental Biology Lab, School of Biology, College of Science, University of Tehran, Tehran, Iran.
University of Tehran · IRAustralian Regenerative Medicine Institute · AUNational Institute of Genetic Engineering and Biotechnology · IRUniversity of Freiburg · DE

Funding

National Center for Genetic Engineering and Biotechnology 1917
6 · The paper itself

Abstract

So far no evidence is available as to whether TGFβ and Wnt signaling pathways cooperatively modulate dopaminergic differentiation of the adult stem cells. To investigate the interaction between the two pathways in early dopaminergic differentiation, we cultured the newly introduced unrestricted somatic stem cells (USSCs) in neuron differentiation media followed by treatments with inducers and inhibitors of Wnt and TGF beta pathways either alone or in combinations. Our results showed that the level of Nurr-1 as a marker for dopaminergic neuron precursors and that of the nuclear β-catenin as the key effector of the active Wnt pathway were significantly elevated following the treatment with either TGFβ or BIO (the Wnt pathway inducer). Conversely, Nurr-1 expression was significantly reduced following the combined treatments with SB431542 (the TGFβ inhibitor) plus BIO or with TGFβ plus Dkk1 (the specific Wnt inhibitor). Nuclear β-catenin was also significantly reduced following combined treatments with SB431542 plus either BIO or TGFβ. Altogether, our results imply that Wnt and TGFβ signaling pathways cooperatively ensure the early dopaminergic differentiation of the USSC adult stem cells.

Indexed as

NeurogenesisWnt Signaling PathwayBenzamidesbeta CateninCells, CulturedDioxolesDopaminergic NeuronsFetal BloodHumansMesenchymal Stem CellsNuclear Receptor Subfamily 4, Group A, Member 2Transforming Growth Factor beta4-(5-benzo(1,3)dioxol-5-yl-4-pyridin-2-yl-1H-imidazol-2-yl)benzamideBenzamidesbeta CateninDioxolesNR4A2 protein, humanNuclear Receptor Subfamily 4, Group A, Member 2Transforming Growth Factor betaDopaminergic neural differentiationTGFβUnrestricted somatic stem cellsWnt

Identifiers

PMID32043204
OpenAlexW3006024000

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.