ArticleHeliyon2020
Improving sensitivity of single tube nested PCR to detect fastidious microorganisms.
Article in Heliyon, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Multi-year evaluation of genetic variability and population structure in bell pepper using integrated phenotypic traits and SSR marker data.Journal of applied genetics · 2026Article
- One-pot electrochemical detection of foodborne pathogen based on in situ nucleic acid amplification and wash-free assay.Mikrochimica acta · 2024Article
- Molecular diagnosis of human toxoplasmosis: the state of the art.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- Development of a novel sensitive single-tube nested PCR assay for the detection of African swine fever virus.Archives of virology · 2024Article
- Application of a modified tetra-primer ARMS-PCR assay for rapid Panax species identity authentication in ginseng products.Scientific reports · 2023Article
- Are ELISA and PCR Discrepancies in the Identification ofMicroorganisms · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Single Tube Nested PCR (ST-nPCR) is of value to clinical laboratories with limited settings for the detection of fastidious microorganisms. The detection sensitivity of ST-nPCR is dependent on ensuring minimal leftovers of outer primers during the second round of the reaction. In this work, we investigated various approaches to optimize the performance of outer primers, including decreasing outer primer concentrations; using antisense oligonucleotides to block outer primers; using chemically modified inner primers; and using Q5 Taq polymerase that lacks 5'-3' exonuclease and strand displacement capabilities. These solutions were tested on
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.