ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2020
Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle-Resolved Subvoxel SPIM.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 28 citations in OpenAlex.
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- Whole-brain Optical Imaging: A Powerful Tool for Precise Brain Mapping at the Mesoscopic Level.Neuroscience bulletin · 2023Review
- Light sheet imaging and interactive analysis of the cardiac structure in neonatal mice.Journal of biophotonics · 2023Article
- Three-dimensional mapping in multi-samples with large-scale imaging and multiplexed post staining.Communications biology · 2023Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Advanced high resolution three-dimensional imaging to visualize the cerebral neurovascular network in stroke.International journal of biological sciences · 2022Review
- Three-dimensional reconstruction of testis cords/seminiferous tubules.Reproductive medicine and biology · 2021Review
- FDISCO+: a clearing method for robust fluorescence preservation of cleared samples.Neurophotonics · 2021Article
- Urea-based amino sugar agent clears murine liver and preserves protein fluorescence and lipophilic dyes.BioTechniques · 2021Article
- Article
- Optical Clearing and Light Sheet Microscopy Imaging of Amphioxus.Frontiers in cell and developmental biology · 2021Article
- Deep-learning super-resolution light-sheet add-on microscopy (Deep-SLAM) for easy isotropic volumetric imaging of large biological specimens.Biomedical optics express · 2020Article
- Axial resolution improvement of two-photon microscopy by multi-frame reconstruction and adaptive optics.Biomedical optics express · 2020Article
- Fast, 3D Isotropic Imaging of Whole Mouse Brain Using Multiangle-Resolved Subvoxel SPIM.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2020Article
Corrections and comments
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Authors and funding
12 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The recent integration of light-sheet microscopy and tissue-clearing has facilitated an important alternative to conventional histological imaging approaches. However, the in toto cellular mapping of neural circuits throughout an intact mouse brain remains highly challenging, requiring complicated mechanical stitching, and suffering from anisotropic resolution insufficient for high-quality reconstruction in 3D. Here, the use of a multiangle-resolved subvoxel selective plane illumination microscope (Mars-SPIM) is proposed to achieve high-throughput imaging of whole mouse brain at isotropic cellular resolution. This light-sheet imaging technique can computationally improve the spatial resolution over six times under a large field of view, eliminating the use of slow tile stitching. Furthermore, it can recover complete structural information of the sample from images subject to thick-tissue scattering/attenuation. With Mars-SPIM, a digital atlas of a cleared whole mouse brain (≈7 mm × 9.5 mm × 5 mm) can readily be obtained with an isotropic resolution of ≈2 µm (1 µm voxel) and a short acquisition time of 30 min. It provides an efficient way to implement system-level cellular analysis, such as the mapping of different neuron populations and tracing of long-distance neural projections over the entire brain. Mars-SPIM is thus well suited for high-throughput cell-profiling phenotyping of brain and other mammalian organs.
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