ArticlePlant methods2020
Cut and paste: temperature-enhanced cotyledon micrografting for
Article in Plant methods, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- High-Throughput Hypocotyl Grafting and Vascular Reconnection Assessment in Arabidopsis Seedlings.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Integrative Analyses of Metabolome and Transcriptome Reveal Scion-Stock Asymmetry Reduction and Shift of Sugar Metabolism During Graft Junction Formation in Malus Domestica ('Hanfu') Homograft.International journal of molecular sciences · 2025Article
- Here comes the sun: integration of light, temperature, and auxin during herbaceous plant grafting.Planta · 2025Review
- Monitoring Xylem Transport in Arabidopsis thaliana Seedlings Using Fluorescent Dyes.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Vacuolar Sugar Transporter TMT2 Plays Crucial Roles in Germination and Seedling Development in Arabidopsis.International journal of molecular sciences · 2023Article
- High temperature perception in leaves promotes vascular regeneration and graft formation in distant tissues.Development (Cambridge, England) · 2022Article
- Insights Into Plant Surgery: An Overview of the Multiple Grafting Techniques forFrontiers in plant science · 2020Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCotyledon micrografting represents a useful tool for studying the central role of cotyledons during early plant development, especially their interplay with other plant organs with regard to long distance transport. While hypocotyl micrografting methods are well-established, cotyledon micrografting is still inefficient. By optimizing cotyledon micrografting, we aim for higher success rates and increased throughput in the model species
resultsWe established a cut and paste cotyledon surgery procedure on a flat and solid but moist surface which improved handling of small seedlings. By applying a specific cutting and joining pattern, throughput was increased up to 40 seedlings per hour. The combination of short-day photoperiods and low light intensities for germination and long days plus high light intensities, elevated temperature and vertical plate positioning after grafting significantly increased 'ligation' efficiency. In particular high temperatures affected success rates favorably. Altogether, we achieved up to 92% grafting success in
conclusionsThis cut and paste cotyledon-to-petiole micrografting protocol simplifies the handling of plant seedlings in surgery, increases the number of grafted plants per hour and greatly improves success rates for
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.