Evidence map›Paper›PMID 32042275›Full record

ArticleJournal of biomolecular techniques : JBT2020

Selection of a Noninvasive Source of Human DNA Envisaging Genotyping Assays in Epidemiological Studies: Urine or Saliva?

Sarah J D Nauwelaerts, Dirk Van Geel, Maud Delvoye, Koen De Cremer, Alfred Bernard, Nancy H C Roosens, Sigrid C J De Keersmaecker

Abstract read
In one paragraph

Article in Journal of biomolecular techniques : JBT, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Sarah J D NauwelaertsTransversal Activities in Applied Genomics, Sciensano, 1050 Brussels, Belgium.
Dirk Van GeelTransversal Activities in Applied Genomics, Sciensano, 1050 Brussels, Belgium.
Maud DelvoyeTransversal Activities in Applied Genomics, Sciensano, 1050 Brussels, Belgium.
Koen De CremerPlatform Chromatography and Mass Spectrometry, Sciensano, 1050 Brussels, Belgium; and.
Alfred BernardLouvain Centre for Toxicology and Applied Pharmacology, Université Catholique de Louvain Woluwe, 1200 Brussels, Belgium.
Nancy H C RoosensTransversal Activities in Applied Genomics, Sciensano, 1050 Brussels, Belgium.
Sigrid C J De KeersmaeckerTransversal Activities in Applied Genomics, Sciensano, 1050 Brussels, Belgium.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genetic epidemiology requires an appropriate approach to measure genetic variation within the population. The aim of this study was to evaluate the characteristics and genotyping results of DNA extracted from 2 human DNA sources, selected for their rapid and noninvasive sampling, and the use of simple and standardized protocols that are essential for large-scale epidemiologic studies. Saliva and urine samples were collected at the same day from 20 subjects aged 9-10 yr. Genomic DNA was extracted using commercial kits. Quantitative and qualitative evaluation was done by assessing the yield, the purity, and integrity of the extracted DNA. As a proof-of-concept, genotyping was performed targeting

Indexed as

BiomarkersBody FluidsChildDNAFemaleGenotypeGenotyping TechniquesHumansMaleMolecular EpidemiologyPolymerase Chain ReactionPolymorphism, Single NucleotideSalivaSecretoglobinsSpecimen HandlingUteroglobinBiomarkersDNASCGB1A1 protein, humanSCGB3A2 protein, humanSecretoglobinsUteroglobinbiomarkers, genotype, real-time PCR, genetic epidemiology, children

Identifiers

PMID32042275
PMCPMC6977458

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.