ArticleNature protocols2020
Long-term in vivo imaging of Drosophila larvae.
Article in Nature protocols, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Biolipid Film-Fused Electrochemiluminescence for Multipurpose In Situ Bioassays.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Spatial microenvironments tune immune response dynamics in the Drosophila larval fat body.PLoS genetics · 2026Article
- Spatial microenvironments tune immune response dynamics in thebioRxiv : the preprint server for biology · 2025Article
- Muscle growth by sarcomere divisions.Science advances · 2025Article
- Immobilizing Second-InstarCold Spring Harbor protocols · 2025Article
- Retromer promotes the lysosomal turnover of mtDNA.Science advances · 2025Article
- Thelaziasis in an urban woman in Beijing: a case report and literature review.BMC ophthalmology · 2024Review
- Developmental emergence of sleep rhythms enables long-term memory inScience advances · 2023Article
- Kinetics of blood cell differentiation during hematopoiesis revealed by quantitative long-term live imaging.eLife · 2023Article
- Article
- Activating RAC1 variants in the switch II region cause a developmental syndrome and alter neuronal morphology.Brain : a journal of neurology · 2022Article
- Hemocytes are essential for Drosophila melanogaster post-embryonic development, independent of control of the microbiota.Development (Cambridge, England) · 2022Article
- Flexible method for generating needle-shaped beams and its application in optical coherence tomography.Optica · 2022Article
- Autophagy-mediated plasma membrane removal promotes the formation of epithelial syncytia.The EMBO journal · 2022Article
- Distinct responses to rare codons in selecteLife · 2022Article
- Current methods to analyze lysosome morphology, positioning, motility and function.Traffic (Copenhagen, Denmark) · 2022Review
- Rapid and robust optogenetic control of gene expression in Drosophila.Developmental cell · 2021Article
- miR-1 sustains muscle physiology by controlling V-ATPase complex assembly.Science advances · 2021Article
- Analyzing muscle structure and function throughout the larval instars in liveSTAR protocols · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Drosophila larva has been used to investigate many processes in cell biology, including morphogenesis, physiology and responses to drugs and new therapeutic compounds. Despite its enormous potential as a model system, longer-term live imaging has been technically challenging because of a lack of efficient methods for immobilizing larvae for extended periods. We describe here a simple procedure for anesthetization and uninterrupted long-term in vivo imaging of the epidermis and other larval organs, including gut, imaginal discs, neurons, fat body, tracheae, muscles and hemocytes, for up to 8 h. We also include a procedure for probing cell properties by laser ablation. We provide a survey of the effects of different anesthetics, demonstrating that short exposure to diethyl ether is the most effective for long-term immobilization of larvae. This protocol does not require specific expertise beyond basic Drosophila genetics and husbandry, and confocal microscopy. It enables high-resolution studies of many systemic and subcellular processes in larvae.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.