ArticleeLife2020
High-dimensional analysis of intestinal immune cells during helminth infection.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 33 citations in OpenAlex.
- Progenitor T cells drive chronic pulmonary type 2 inflammation.Nature immunology · 2026Article
- Crotoxin fromFrontiers in immunology · 2026Article
- Dominant immune tolerance in the intestinal tract imposed by RelB-dependent migratory dendritic cells regulates protective type 2 immunity.Nature communications · 2024Article
- Enteric tuft cells coordinate timely expulsion of the tapeworm Hymenolepis diminuta from the murine host by coordinating local but not systemic immunity.PLoS pathogens · 2024Article
- OMIP-095: 40-Color spectral flow cytometry delineates all major leukocyte populations in murine lymphoid tissues.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2023Article
- High-Throughput Single-Cell Analysis of Nanoparticle-Cell Interactions.Trends in analytical chemistry : TRAC · 2023Article
- Resident TScience immunology · 2022Article
- Tissue-specific immunity in helminth infections.Mucosal immunology · 2022Review
- Tissue-based IL-10 signalling in helminth infection limits IFNγ expression and promotes the intestinal Th2 response.Mucosal immunology · 2022Article
- Epithelial STAT6 O-GlcNAcylation drives a concerted anti-helminth alarmin response dependent on tuft cell hyperplasia and Gasdermin C.Immunity · 2022Article
- IL-33 and the PKA Pathway Regulate ILC2 Populations Expressing IL-9 and ST2.Frontiers in immunology · 2022Article
- Intestinal eosinophils: multifaceted roles in tissue homeostasis and disease.Seminars in immunopathology · 2021Review
- Interrogating the Small Intestine Tuft Cell-ILC2 Circuit Using In Vivo Manipulations.Current protocols · 2021Article
- Bifidobacterium bifidum strains synergize with immune checkpoint inhibitors to reduce tumour burden in mice.Nature microbiology · 2021Article
- Article
- High-Dimensional Data Analysis Algorithms Yield Comparable Results for Mass Cytometry and Spectral Flow Cytometry Data.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2020Article
- Isolation and functional characterisation of lamina propria leukocytes from helminth-infected, murine small intestine.Journal of immunological methods · 2020Article
- Defined Intestinal Regions Are Drained by Specific Lymph Nodes That Mount Distinct Th1 and Th2 Responses AgainstFrontiers in immunology · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Single cell isolation from helminth-infected murine intestines has been notoriously difficult, due to the strong anti-parasite type 2 immune responses that drive mucus production, tissue remodeling and immune cell infiltration. Through the systematic optimization of a standard intestinal digestion protocol, we were able to successfully isolate millions of immune cells from the heavily infected duodenum. To validate that these cells gave an accurate representation of intestinal immune responses, we analyzed them using a high-dimensional spectral flow cytometry panel and confirmed our findings by confocal microscopy. Our cell isolation protocol and high-dimensional analysis allowed us to identify many known hallmarks of anti-parasite immune responses throughout the entire course of helminth infection and has the potential to accelerate single-cell discoveries of local helminth immune responses that have previously been unfeasible.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.