ArticleeLife2020
Imaging plant germline differentiation within Arabidopsis flowers by light sheet microscopy.
Article in eLife, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- A simple hand-sectioning approach for cellular-resolution imaging of anatomy and gene expression in small developing organs and boundary regions in Arabidopsis.Plant methods · 2026Article
- SMG7 and eIF4A constitute a homeostatic module controlling P-body condensation and function of meiotic bodies.Nature communications · 2026Article
- Pre-meiotic H1.1 degradation is essential for Arabidopsis gametogenesis.The EMBO journal · 2026Article
- Actin filaments drive spindle positioning in Arabidopsis meiosis II.The New phytologist · 2025Article
- VR-prep improves visualization of medical data in augmented reality on smartphones.Scientific reports · 2025Article
- A Protocol for Live Imaging of Arabidopsis Gynoecium Development Using Confocal Microscopy.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Time-lapse confocal imaging helps to reveal a secret behind gynoecium development.Quantitative plant biology · 2025Article
- Applications of Lightsheet Fluorescence Microscopy by High Numerical Aperture Detection Lens.The journal of physical chemistry. B · 2024Review
- Rapid meiotic prophase chromosome movements in Arabidopsis thaliana are linked to essential reorganization at the nuclear envelope.Nature communications · 2024Article
- Meiotic double-strand break repair DNA synthesis tracts in Arabidopsis thaliana.PLoS genetics · 2024Article
- Article
- Observing ER Dynamics over Long Timescales Using Light Sheet Fluorescence Microscopy.Methods in molecular biology (Clifton, N.J.) · 2024Article
- OME-Zarr: a cloud-optimized bioimaging file format with international community support.Histochemistry and cell biology · 2023Article
- Advanced microscopy resolves dynamic localization patterns of stress-induced mitogen-activated protein kinase (SIMK) during alfalfa root hair interactions with Ensifer meliloti.Journal of experimental botany · 2023Article
- OME-Zarr: a cloud-optimized bioimaging file format with international community support.bioRxiv : the preprint server for biology · 2023Article
- Ascorbic acid-mediated reactive oxygen species homeostasis modulates the switch from tapetal cell division to cell differentiation in Arabidopsis.The Plant cell · 2023Article
- TurboID-based proteomic profiling of meiotic chromosome axes in Arabidopsis thaliana.Nature plants · 2023Article
- A complex role of Arabidopsis CDKD;3 in meiotic progression and cytokinesis.Plant direct · 2023Article
- Fluorescent Fusion Protein Expression in Plant Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Meiotic chromosome organization and its role in recombination and cancer.Current topics in developmental biology · 2023Review
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9 authors.
Funding
Abstract
In higher plants, germline differentiation occurs during a relatively short period within developing flowers. Understanding of the mechanisms that govern germline differentiation lags behind other plant developmental processes. This is largely because the germline is restricted to relatively few cells buried deep within floral tissues, which makes them difficult to study. To overcome this limitation, we have developed a methodology for live imaging of the germ cell lineage within floral organs of Arabidopsis using light sheet fluorescence microscopy. We have established reporter lines, cultivation conditions, and imaging protocols for high-resolution microscopy of developing flowers continuously for up to several days. We used multiview imagining to reconstruct a three-dimensional model of a flower at subcellular resolution. We demonstrate the power of this approach by capturing male and female meiosis, asymmetric pollen division, movement of meiotic chromosomes, and unusual restitution mitosis in tapetum cells. This method will enable new avenues of research into plant sexual reproduction.
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