ArticleParasites & vectors2020
Wolbachia strain wAlbB blocks replication of flaviviruses and alphaviruses in mosquito cell culture.
Article in Parasites & vectors, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Trial
- Natural infection of Aedes albopictus with the wAlbB strain and Ae. aegypti with the wPip strain of Wolbachia in Iran.Scientific reports · 2026Article
- Bacterial community structure and diversity of common mosquito species in Chengdu: Insights from PacBio third-generation sequencing and public health implications.PLoS neglected tropical diseases · 2026Article
- The inhibition mechanism ofFrontiers in microbiology · 2026Review
- Article
- The interplay between vector microbial community and pathogen transmission on the invasive Asian tiger mosquito,Frontiers in microbiology · 2023Review
- NativeFrontiers in microbiology · 2023Article
- Lab-scale characterization and semi-field trials of Wolbachia Strain wAlbB in a Taiwan Wolbachia introgressed Ae. aegypti strain.PLoS neglected tropical diseases · 2022Article
- High Temperature Cycles Result in Maternal Transmission and Dengue Infection Differences BetweenmBio · 2021Article
- Article
- Releasing incompatible males drives strong suppression across populations of wild andProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Isolation in Natural Host Cell Lines ofInsects · 2021Article
- Growth and Maintenance ofInsects · 2021Review
- Wolbachia: endosymbiont of onchocercid nematodes and their vectors.Parasites & vectors · 2021Review
- Bacterial Symbionts of Tsetse Flies: Relationships and Functional Interactions Between Tsetse Flies and Their Symbionts.Results and problems in cell differentiation · 2020Review
- Historical Perspective and Biotechnological Trends to Block Arboviruses Transmission by ControllingFrontiers in medicine · 2020Review
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Authors and funding
4 authors.
Funding
Abstract
backgroundWolbachia pipientis are bacterial endosymbionts of arthropods currently being implemented as biocontrol agents to reduce the global burden of arboviral diseases. Some strains of Wolbachia, when introduced into Aedes aegypti mosquitoes, reduce or block the replication of RNA viruses pathogenic to humans. The wAlbB strain of Wolbachia was originally isolated from Aedes albopictus, and when transinfected into Ae. aegypti, persists in mosquitoes under high temperature conditions longer than other strains. The utility of wAlbB to block a broad spectrum of RNA viruses has received limited attention. Here we test the ability of wAlbB to reduce or block the replication of a range of Flavivirus and Alphavirus species in cell culture.
methodsThe C6/36 mosquito cell line was stably infected with the wAlbB strain using the shell-vial technique. The replication of dengue, West Nile and three strains of Zika (genus Flavivirus), and Ross River, Barmah Forest and Sindbis (genus Alphavirus) viruses was compared in wAlbB-infected cells with Wolbachia-free controls. Infectious virus titres were determined using either immunofocus or plaque assays. A general linear model was used to test for significant differences in replication between flaviviruses and alphaviruses.
resultsTitres of all viruses were significantly reduced in cell cultures infected with wAlbB versus Wolbachia-free controls. The magnitude of reduction in virus yields varied among virus species and, within species, also among the strains utilized.
conclusionOur results suggest that wAlbB infection of arthropods could be used to reduce transmission of a wide range of pathogenic RNA viruses.
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