ArticleThe Journal of cell biology1988
The dynamic distribution of fluorescent analogues of actin and myosin in protrusions at the leading edge of migrating Swiss 3T3 fibroblasts.
Article in The Journal of cell biology, 1988. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
36 citing papers in PubMed, 101 citations in OpenAlex.
- Identification and characterization of Myosin from rat testicular peritubular myoid cells.Biology of reproduction · 2008Article
- The C-terminal tail region of nonmuscle myosin II directs isoform-specific distribution in migrating cells.Molecular biology of the cell · 2008Article
- Retrograde flow and myosin II activity within the leading cell edge deliver F-actin to the lamella to seed the formation of graded polarity actomyosin II filament bundles in migrating fibroblasts.Molecular biology of the cell · 2008Article
- Regulation of myosin II dynamics by phosphorylation and dephosphorylation of its light chain in epithelial cells.Molecular biology of the cell · 2007Article
- The role of myosin II motor activity in distributing myosin asymmetrically and coupling protrusive activity to cell translocation.Molecular biology of the cell · 2006Article
- Asymmetric distribution of myosin IIB in migrating endothelial cells is regulated by a rho-dependent kinase and contributes to tail retraction.Molecular biology of the cell · 2003Article
- A fluorescent resonant energy transfer-based biosensor reveals transient and regional myosin light chain kinase activation in lamella and cleavage furrows.The Journal of cell biology · 2002Article
- Myosin II dynamics and cortical flow during contractile ring formation in Dictyostelium cells.The Journal of cell biology · 2001Article
- An analysis of actin delivery in the acrosomal process of thyone.Biophysical journal · 1999Article
- Two components of actin-based retrograde flow in sea urchin coelomocytes.Molecular biology of the cell · 1999Article
- Keratocytes generate traction forces in two phases.Molecular biology of the cell · 1999Article
- The actin-based nanomachine at the leading edge of migrating cells.Biophysical journal · 1999Article
- Intracellular fluorescent probe concentrations by confocal microscopy.Biophysical journal · 1998Article
- Luminescence resonance energy transfer measurements in myosin.Biophysical journal · 1998Article
- Myogenic cells express multiple myosin isoforms.Journal of muscle research and cell motility · 1997Article
- AOTF microscope for imaging with increased speed and spectral versatility.Biophysical journal · 1997Article
- Role of actin polymerization and adhesion to extracellular matrix in Rac- and Rho-induced cytoskeletal reorganization.The Journal of cell biology · 1997Article
- Cell motility driven by actin polymerization.Biophysical journal · 1996Article
- Myosin II transport, organization, and phosphorylation: evidence for cortical flow/solation-contraction coupling during cytokinesis and cell locomotion.Molecular biology of the cell · 1996Article
- A fluorescent protein biosensor of myosin II regulatory light chain phosphorylation reports a gradient of phosphorylated myosin II in migrating cells.Molecular biology of the cell · 1995Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The formation of protrusions at the leading edge of the cell is an essential step in fibroblast locomotion. Using fluorescent analogue cytochemistry, ratio imaging, multiple parameter analysis, and fluorescence photobleaching recovery, the distribution of actin and myosin was examined in the same protrusions at the leading edge of live, locomoting cells during wound-healing in vitro. We have previously defined two temporal stages of the formation of protrusions: (a) initial protrusion and (b) established protrusion (Fisher et al., 1988). Actin was slightly concentrated in initial protrusions, while myosin was either totally absent or present at extremely low levels at the base of the initial protrusions. In contrast, established protrusions contained diffuse actin and actin microspikes, as well as myosin in both diffuse and structured forms. Actin and myosin were also localized along concave transverse fibers near the base of initial and established protrusions. The dynamics of myosin penetration into a relatively stable, established protrusion was demonstrated by recording sequential images over time. Myosin was shown to be absent from an initial protrusion, but diffuse and punctate myosin was detected in the same protrusion within 1-2 min. Fluorescence photobleaching recovery indicated that myosin was 100% immobile in the region behind the leading edge containing transverse fibers, in comparison to the 21% immobile fraction detected in the perinuclear region. Possible explanations of the delayed penetration of myosin into established protrusions and the implications on the mechanism of protrusion are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.