ArticlePloS one2020
Serological and molecular investigation of hepatitis E virus in pigs reservoirs from Cameroon reveals elevated seroprevalence and presence of genotype 3.
Article in PloS one, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 2 of them syntheses that pooled it.
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Who cites it
16 citing papers in PubMed, 2 syntheses or guidelines pooled it, 24 citations in OpenAlex.
- Hepatitis E Virus (HEV) Infection in the Context of the One Health Approach: A Systematic Review.Pathogens (Basel, Switzerland) · 2025Pooled it
- Epidemiology of hepatitis E virus infection in animals in Africa: a systematic review and meta-analysis.BMC veterinary research · 2021Pooled it
- Article
- Molecular characterization of enteroviruses circulating among pigs and goats in two Central African countries, Cameroon and the Central African Republic.Access microbiology · 2025Article
- Detection of Hepatitis E Virus (HEV) in Pork Sold in Saint-Louis, the North of Senegal.Life (Basel, Switzerland) · 2024Article
- Hepatitis E Seroprevalence and Detection of Genotype 3 Strains in Domestic Pigs from Sierra Leone Collected in 2016 and 2017.Viruses · 2024Article
- Zoonotic and Food-Related Hazards Due to Hepatitis A and E in Africa: A Systematic Review and Meta-Analysis.Environmental health insights · 2024Review
- Article
- Investigating animal reservoirs for hepatitis E virus in Bangui, Central African Republic.PloS one · 2024Article
- Hepatitis E Virus in Livestock-Update on Its Epidemiology and Risk of Infection to Humans.Animals : an open access journal from MDPI · 2023Review
- Hepatitis E Virus (HEV) in Heavy Pigs in Slaughterhouses of Northern Italy: Investigation of Seroprevalence, Viraemia, and Faecal Shedding.Animals : an open access journal from MDPI · 2023Article
- First Detection and Characterization of Hepatitis E Virus in Sewage Samples in Cameroon.Food and environmental virology · 2023Article
- Characterization of a Near Full-Length Hepatitis E Virus Genome of Subtype 3c Generated from Naturally Infected South African Backyard Pigs.Pathogens (Basel, Switzerland) · 2022Article
- Nationwide survey of hepatitis E virus infection among wildlife in Japan.The Journal of veterinary medical science · 2022Article
- Prevalence of hepatitis E virus antibodies in cattle in Burkina Faso associated with swine mixed farming.Journal of veterinary science · 2022Article
- First molecular detection of hepatitis E virus genome in camel and pig faecal samples in Ethiopia.Virology journal · 2021Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHepatitis E virus (HEV) is a zoonotic pathogen of which pigs have been established as reservoirs. In the present study, we investigated the presence of HEV among pigs in the Center and Littoral regions of Cameroon and performed the molecular characterization of positive strains. METHODOLOGY: A total of 453 serum and stool samples were randomly collected from pigs in slaughterhouses in Obala, Douala and Yaounde. All samples were examined for the presence of anti-HEV IgG and IgM antibodies using ELISA assays. IgM positive stool samples were tested for HEV RNA using an RT-PCR assay, followed by a nested PCR assay for sequencing and phylogenetic analysis.
resultsOverall, 216 samples (47.7%, 95% CI: 43.1%-52.3%) were positive for at least one of the serological markers of HEV infection. Amongst these, 21.0% were positives for anti-HEV IgM, 17.7% for anti-HEV IgG, and 9.1% for both. A total of eight stool samples (5.9%) were positive for HEV RNA by nested RT-PCR. Phylogenetic analysis showed that the retrieved sequences clustered within HEV genotype 3.
conclusionThis study shows a high prevalence of anti-HEV antibodies and the circulation of genotype 3 in the swine population in Cameroon. Subsequent studies will be needed to elucidate the zoonotic transmission of HEV from pigs to humans in Cameroon.
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