ArticleStem cell investigation2019
Evaluation of platelet-rich plasma from diabetic donors shows increased platelet vascular endothelial growth factor release.
Article in Stem cell investigation, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 18 citations in OpenAlex.
- The application of platelet-rich plasma for skin graft enrichment: A meta-analysis.International wound journal · 2020Pooled it
- Nutritional Interventions to Optimize Orthobiologic Therapy Quality in Type 2 Diabetes Mellitus: Molecular Mechanisms and Clinical Framework: A Narrative Review.International journal of molecular sciences · 2026Review
- Dietary Patterns Are Associated with Blood Cell Profiles and the Molecular Composition of Platelet-Rich Plasma.Nutrients · 2026Article
- Effect of glycaemic control on growth factor release andFrontiers in dental medicine · 2026Article
- Refrigerated storage of L-PRF membranes: anti-bacterial activity, cytokine release, andFrontiers in oral health · 2026Article
- Does Diabetes Matter? The Efficacy of PRP on the Quality of Life in Stress Urinary Incontinence.Bioengineering (Basel, Switzerland) · 2025Article
- Review
- Biological processes and factors involved in soft and hard tissue healing.Periodontology 2000 · 2025Review
- Platelet-rich plasma may accelerate diabetic wound healing by modulating epithelial/endothelial-mesenchymal transition through inhibiting reactive oxygen species-mediated oxidative stress.Frontiers in bioengineering and biotechnology · 2025Article
- The Effect of Intravenous Autologous Activated Platelet-Rich Plasma Therapy on "Profibrotic Cytokine" IL-1Scientifica · 2021Article
- Phase I/II Clinical Trial of Autologous Activated Platelet-Rich Plasma (aaPRP) in the Treatment of Severe Coronavirus Disease 2019 (COVID-19) Patients.International journal of inflammation · 2021Article
- Safety of Technique and Procedure of Stromal Vascular Fraction Therapy: From Liposuction to Cell Administration.Scientifica · 2020Article
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Authors and funding
11 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPlatelet-rich plasma (PRP) contains pro-angiogenic growth factors including vascular endothelial growth factor (VEGF). Angiogenesis is a necessary component of wound healing in instances of diabetic foot ulcers (DFU). PRP composition varies depending on methods and donor health status. Our group has developed an improved PRP protocol for diabetes treatment. The aims of this study were to examine the levels of the pro-angiogenic factor VEGF in these patient populations with and without diabetes.
methodsPRP was prepared using 24 mL of whole blood from 13 diabetic and 10 non-diabetic patients registered at Klinik Hayandra. Whole blood in sodium citrate tubes were centrifuged at 1,000 rpm for 5 minutes followed by plasma separation. Plasma samples were centrifuged at 3,000 rpm for 5 minutes. Upper platelet-poor plasma layers were discarded, leaving 5 mL of concentrated platelet containing plasma (PRP). Concentrated plasma samples were mixed, aliquoted, stored at -86 °C, and pooled for platelet count, VEGF, and total protein analyses. Platelet counting was also performed using fresh whole blood and PRP to measure changes following PRP preparation.
resultsDiabetic donors had higher whole blood platelet counts than non-diabetic donors, but this difference was not statistically significant. An average increase of more than 250% in platelet number after PRP preparation using our method was noted in both groups. Freezing-thawing samples at -86 °C lysed more than 90% of PRP platelets regardless of diabetes status. Diabetic PRP had lower mean total protein and higher VEGF concentrations. Lysed platelets from diabetic donors released more VEGF than those from non-diabetic donors.
conclusionsPRP from diabetic donors had higher VEGF content making autologous PRP application a promising treatment for DFU. However, this should be investigated another appropriate clinical trial.
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