ArticleFrontiers in cell and developmental biology2019
Membrane Potential Determined by Flow Cytometry Predicts Fertilizing Ability of Human Sperm.
Article in Frontiers in cell and developmental biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 52 citations in OpenAlex.
- Systematic review and meta-analysis of cryopreserved bovine sperm assessment: harnessing imaging flow cytometry for multi-parametric analysis.Frontiers in veterinary science · 2024Pooled it
- Sperm capacitation ability, as evaluated by Cap-Score™, has little impact on ICSI success.Journal of assisted reproduction and genetics · 2026Article
- Sperm plasma membrane potential as a marker for fertilization success for non-normozoospermic patients undergoing assisted reproduction technologies.Journal of assisted reproduction and genetics · 2026Article
- Correlation of human sperm intracellular pH in non-normozoospermic men with fertilization rates in assisted reproduction procedures.Journal of assisted reproduction and genetics · 2026Article
- Membrane potential and feedback dynamics regulate CatSper-mediated progesterone signaling in human sperm.bioRxiv : the preprint server for biology · 2025Article
- Highly Potent and Subtype-Selective Sperm-Specific Potassium Channel SLO3 Inhibitors Display High Tissue Exposure in the Murine Female Reproductive Tract.ACS pharmacology & translational science · 2025Article
- Functional parameters of spermatozoa obtained by a new selection device.FEBS open bio · 2025Article
- Bacterial vaginosis toxins impair sperm capacitation and fertilization.Human reproduction (Oxford, England) · 2025Article
- High Variability in Human Sperm Membrane Potential over Time Can Limit Its Reliability as a Predictor in ART Outcomes.Biology · 2025Article
- Equine in vitro fertilization with frozen-thawed semen is associated with shortened pre-incubation time and modified capacitation-related changes.Biology of reproduction · 2025Article
- Membrane potential hyperpolarization: a critical factor in acrosomal exocytosis and fertilization in sperm within the female reproductive tract.Frontiers in cell and developmental biology · 2024Article
- NaInternational journal of molecular sciences · 2023Review
- Mitochondrial uncouplers impair human sperm motility without altering ATP content†.Biology of reproduction · 2023Article
- SLO3: A Conserved Regulator of Sperm Membrane Potential.International journal of molecular sciences · 2023Review
- The SLC9C2 Gene Product (NaInternational journal of molecular sciences · 2023Article
- A selective inhibitor of the sperm-specific potassium channel SLO3 impairs human sperm function.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Sperm calcium flux and membrane potential hyperpolarization observed in the Mexican big-eared bat Corynorhinus mexicanus.The Journal of experimental biology · 2023Article
- Repair mechanism of Wuwei Fuzheng Yijing formula in di-2-ethylhexyl phthalate-induced sperm DNA fragmentation in mice.Pharmaceutical biology · 2022Article
- Elevated and Sustained Intracellular Calcium Signalling Is Necessary for Efficacious Induction of the Human Sperm Acrosome Reaction.International journal of molecular sciences · 2022Article
- A genetically targeted sensor reveals spatial and temporal dynamics of acrosomal calcium and sperm acrosome exocytosis.The Journal of biological chemistry · 2022Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
Infertility affects 10 to 15% of couples worldwide, with a male factor contributing up to 50% of these cases. The primary tool for diagnosing male infertility is traditional semen analysis, which reveals sperm concentration, morphology, and motility. However, 25% of infertile men are diagnosed as normozoospermic, meaning that, in many cases, normal-appearing sperm fail to fertilize an egg. Thus, new information regarding the mechanisms by which sperm acquire fertilizing ability is needed to develop a clinically feasible test that can predict sperm function failure. An important feature of sperm fertilization capability in many species is plasma membrane hyperpolarization (membrane potential becoming more negative inside) in response to signals from the egg or female genital tract. In mice, this hyperpolarization is necessary for sperm to undergo the changes in motility (hyperactivation) and acrosomal exocytosis required to fertilize an egg. Human sperm also hyperpolarize during capacitation, but the physiological relevance of this event has not been determined. Here, we used flow cytometry combined with a voltage-sensitive fluorescent probe to measure absolute values of human sperm membrane potential. We found that hyperpolarization of human sperm plasma membrane correlated positively with fertilizing ability. Hyperpolarized human sperm had higher
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.