ArticleFrontiers in microbiology2019
Droplet Digital PCR Is an Improved Alternative Method for High-Quality Enumeration of Viable Probiotic Strains.
Article in Frontiers in microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed.
- Article
- Revisiting Strategies for Bacterial Enumeration: The Case for Viable but Nonculturable (VBNC) Cells in Supplement Products.Probiotics and antimicrobial proteins · 2026Review
- Factors affecting the viability of probiotics in various food products: a review of current knowledge.Frontiers in microbiology · 2026Review
- A portable device for probiotic counting and viability assessment based on microfluidic chip and image recognition.iMetaOmics · 2025Article
- Comparison of qRT-PCR and ddPCR for multi-strain probiotic detection after a randomized human clinical trial.Frontiers in microbiology · 2025Article
- Characterization and shelf life of synbiotic drink powder from porang (Journal of food science and technology · 2024Article
- Real-time PCR methods for identification and stability monitoring ofFrontiers in microbiology · 2024Article
- Assessing probiotic viability in mixed species yogurt using a novel propidium monoazide (PMAxx)-quantitative PCR method.Frontiers in microbiology · 2024Article
- Review
- PCR-based analytics of gene therapies using adeno-associated virus vectors: Considerations for cGMP method development.Molecular therapy. Methods & clinical development · 2023Review
- Insights into the enumeration of mixtures of probiotic bacteria by flow cytometry.BMC microbiology · 2023Article
- A Healthy Vaginal Microbiota Remains Stable during Oral Probiotic Supplementation: A Randomised Controlled Trial.Microorganisms · 2023Article
- Real-time polymerase chain reaction methods for strain specific identification and enumeration of strainFrontiers in microbiology · 2022Article
- Viability droplet digital polymerase chain reaction accurately enumerates probiotics and provides insight into damage experienced during storage.Frontiers in microbiology · 2022Article
- Enumeration of Probiotic Strain Lacticaseibacillus rhamnosus GG (ATCC 53103) Using Viability Real-time PCR.Probiotics and antimicrobial proteins · 2021Article
- Microbiological Quality and Resistance to an Artificial Gut Environment of Two Probiotic Formulations.Foods (Basel, Switzerland) · 2021Article
- The International Scientific Association of Probiotics and Prebiotics (ISAPP) consensus statement on the definition and scope of postbiotics.Nature reviews. Gastroenterology & hepatology · 2021Review
- Real-Time PCR Assays for the Specific Identification of Probiotic Strains Lactobacillus gasseri BNR17 and Lactobacillus reuteri LRC (NCIMB 30242).Probiotics and antimicrobial proteins · 2021Article
- Locked Nucleic Acid Hydrolysis Probes for the Specific Identification of Probiotic StrainsFrontiers in microbiology · 2021Article
- Improving and Comparing Probiotic Plate Count Methods by Analytical Procedure Lifecycle Management.Frontiers in microbiology · 2021Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Traditional microbiological enumeration methods have long been employed as the standard evaluation procedure for probiotic microorganisms. These methods are labor intensive, have long-time to results and inherently have a high degree of variability - up to 35%. As clinical probiotic and microbiome science continues to grow and develop, it is increasingly important that researchers thoroughly define and deliver the targeted probiotic dose. Furthermore, to establish high quality commercial products, the same dosage level must be administered to consumers. An ISO method for the use of flow cytometry has been established which does speed up the time to results and reduce variability, but the method has not yet gained widespread adoption across the probiotic industry. This is possibly due to expertise needed to implement and maintain a new testing platform in an established quality system. In this study we compare enumeration using plate counts and flow cytometry to the use of droplet digital PCR (ddPCR), which in addition to giving faster time to results than plate count and less variability than both plate count and flow cytometry, has additional benefits such as strain-specific counts. Use of ddPCR gives the ability to design primers to target deletions and single base pair differences which will allow for strain profiling in microbiome analyses. We demonstrate that ddPCR probiotic enumeration results are positively correlated to both plate count and flow cytometry results and should be considered a viable, next generation enumeration method for the evaluation of probiotics.
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