ArticlePoultry science2020
The effects of dietary Bacillus subtilis supplementation, as an alternative to antibiotics, on growth performance, intestinal immunity, and epithelial barrier integrity in broiler chickens infected with Eimeria maxima.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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Who cites it
52 citing papers in PubMed, 90 citations in OpenAlex.
- Bacillus Subtilis (BG01-4Nutrients · 2023Trial
- Investigation of the Immunomodulatory Effects of Combined Prebiotic, Probiotic, and Postbiotic Mixtures in Chicken Ileal Explant Cultures.Animals : an open access journal from MDPI · 2026Article
- Combination Effects ofBiology · 2026Article
- Bacillus-based probiotics and fermented products as alternatives to antibiotic growth promoters in poultry: Current perspectives.Poultry science · 2026Review
- Recombinant Bacillus subtilis Displaying DHAV-1 VP1 Protein as a Dual-Function Probiotic: Evaluation of Immunological Efficacy, Growth Performance, Antioxidant Capacity, and Intestinal Health in Cherry Valley Ducks.Probiotics and antimicrobial proteins · 2026Article
- Evaluation of dietary Bacillus-based commercial probiotics in broiler chickens.Journal of animal science · 2026Article
- Alginate oligosaccharides derived from tropical brown seaweeds as sustainable alternatives to antibiotic growth promoters in poultry nutrition: Functional mechanisms and production perspectives.Veterinary world · 2026Review
- Exploring the interplay betweenThe veterinary quarterly · 2025Review
- Lysozyme and Bacillus subtilis improve growth performance, nutrient utilization, physiological stress indices, intestinal integrity, and gene expression of heat-stressed broilers.Scientific reports · 2025Article
- The combination of dietary active dry yeast andAnimal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Advancing Nigerian Indigenous Poultry Health and Production, Use of Probiotics as Viable Alternatives to Antibiotics: A Review.Antibiotics (Basel, Switzerland) · 2025Review
- Single or Combined Supplementation ofVeterinary sciences · 2025Article
- Effect of β-Alanine Metabolite on Gut Integrity and Immunity in Commercial Broiler Chickens Infected withAnimals : an open access journal from MDPI · 2024Article
- Important role of Bacillus subtilis as a probiotic and vaccine carrier in animal health maintenance.World journal of microbiology & biotechnology · 2024Review
- Impact of Nanoencapsulated Rosemary Essential Oil as a Novel Feed Additive on Growth Performance, Nutrient Utilization, Carcass Traits, Meat Quality and Gene Expression of Broiler Chicken.Foods (Basel, Switzerland) · 2024Article
- Coccidiosis in Egg-Laying Hens and Potential Nutritional Strategies to Modulate Performance, Gut Health, and Immune Response.Animals : an open access journal from MDPI · 2024Review
- Different levels of single-strain probiotic (Bacillus subtilis) with proteolytic enzyme (serratiopeptidase) can be used as an alternative to antibiotic growth promoters in broiler.Poultry science · 2024Article
- Effects of DietaryAnimals : an open access journal from MDPI · 2023Article
- Development of a new immunodiagnostic tool for poultry coccidiosis using an antigen-capture sandwich assay based on monoclonal antibodies detecting an immunodominant antigen of Eimeria.Poultry science · 2023Article
- Effect of Dietary Organic Selenium on Growth Performance, Gut Health, and Coccidiosis Response in Broiler Chickens.Animals : an open access journal from MDPI · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to investigate the effects of dietary Bacillus subtilis supplementation on growth performance, jejunal lesion scores, oocyst shedding, and cytokine and tight junction protein expression in broiler chickens infected with Eimeria maxima. A total of 196 male day-old Ross 708 broilers were given a nonexperimental diet until 14 D of age. Then, all chickens were randomly assigned to one of seven dietary treatments: 2 basal diets (CON and NC); CON + virginiamycin (AB1); CON + bacitracin methylene disalicylate (BMD; AB2); CON + B. subtilis 1781 (PB1); CON + B. subtilis 747 (PB2); or CON + B. subtilis 1781 + 747 (PB3). At day 21, all chickens except those in the CON group were orally inoculated with E. maxima oocysts. At 7 D after E. maxima infection, the body weight gains of chickens fed PB2 and PB3 increased (P = 0.032) as much as those in chickens fed AB2. The body weight gain and feed efficiency of chickens fed PB2 were significantly increased (P < 0.001), and PB2 chickens showed (P = 0.005) the lowest lesion scores after E. maxima infection. Chickens fed PB2 showed (P < 0.05) lower mRNA expression of IL-1β in infected chicken groups. Chickens in the AB1, AB2, PB1, PB2, and PB3 groups showed (P < 0.05) greater mRNA expression of junctional adhesion molecule 2 in jejunal tissue, whereas occludin expression increased (P < 0.05) in the jejunal tissue of chickens fed AB2 or PB2. Dietary B. subtilis supplementation significantly improved the growth performance of young chickens to a level comparable with that induced by virginiamycin or BMD without E. maxima infection. After infection with E. maxima, dietary virginiamycin and BMD significantly enhanced the epithelial barrier integrity, and the dietary B. subtilis 747 showed significantly enhanced growth performance, intestinal immunity, and epithelial barrier integrity. Together our results indicated that certain strains of B. subtilis provide beneficial effects on the growth of young broiler chickens and have the potential to replace antibiotic growth promoters.
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