ArticlePoultry science2020
Transcriptome analysis and identification of genes associated with chicken sperm storage duration.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Selection Signatures from RAD-Seq Reveal Candidate Genomic Regions Potentially Underlying Immune and Adaptive Traits in Southwest Chinese Local Chickens.Animals : an open access journal from MDPI · 2026Article
- Multi-omics analyses reveal steroid hormone and antioxidant pathways regulating sperm storage capacity in female Muscovy ducks (Cairina moschata).Poultry science · 2026Article
- Transcriptional and epigenetic changes associated with lead exposure in spermatozoa.Asian journal of andrology · 2026Article
- Nociceptive thresholds in broiler chickens are modulated by lameness of their progenitors and sex category.Scientific reports · 2026Article
- Identification of genes associated with sperm storage capacity in hens at different times after insemination by RNA-seq and Ribo-seq.BMC genomics · 2024Article
- Connective Tissue Growth Factor: Regulation, Diseases, and Drug Discovery.International journal of molecular sciences · 2024Review
- MicroRNAs and Their Associated Genes Regulating the Acrosome Reaction in Sperm of High- versus Low-Fertility Holstein Bulls.Animals : an open access journal from MDPI · 2024Article
- Integrative analysis of the ovarian metabolome and transcriptome of the Yaoshan chicken and its improved hybrids.Frontiers in genetics · 2024Article
- Seasonal and age-related changes in sperm quality of farmed arctic charr (Salvelinus alpinus).BMC genomics · 2023Article
- Transcriptome identification of genes associated with uterus-vagina junction epithelial folds formation in chicken hens.Poultry science · 2023Article
- The genomic characteristics affect phenotypic diversity from the perspective of genetic improvement of economic traits.iScience · 2023Article
- Genome-wide mapping of signatures of selection using a high-density array identified candidate genes for growth traits and local adaptation in chickens.Genetics, selection, evolution : GSE · 2023Article
- Identification of candidate genomic regions for egg yolk moisture content based on a genome-wide association study.BMC genomics · 2023Article
- Production performance, egg quality, and uterine gene expression for layers as affected by N-Carbamylglutamate supplementation.Frontiers in veterinary science · 2023Article
- Prolonged repeated inseminations trigger a local immune response and accelerate aging of the uterovaginal junction in turkey hens.Frontiers in physiology · 2023Article
- Turkey hen sperm storage tubule transcriptome response to artificial insemination and the presence of semen.Frontiers in physiology · 2023Article
- Sperm Transcriptome Analysis Accurately Reveals Male Fertility Potential in Livestock.Animals : an open access journal from MDPI · 2022Review
- Whole-genome sequencing reveals the artificial selection and local environmental adaptability of pigeons (Evolutionary applications · 2022Article
- Epididymal mRNA and miRNA transcriptome analyses reveal important genes and miRNAs related to sperm motility in roosters.Poultry science · 2022Article
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The sperm storage tubules located in the mucosal folds of the uterovaginal junction (UVJ) are the primary site of sperm storage in chicken hens after natural mating or artificial insemination (AI). The short-term sperm storage (24 h after mating or AI) in hens was highly associated with immunity and pH-related pathway genes. However, the underlying mechanism of longer duration of sperm storage in female birds remains largely unclear. In the present study, transcriptome analysis was applied to uncover the dynamic gene expression changes in chicken UVJ tissues at two time points (day 3 and day 9) after AI. A total of 574 differentially expressed genes (DEG) were enriched, including 266 upregulated and 308 downregulated DEG. The validation of 5 DEG using quantitative PCR showed a similar expression tendency with RNA sequencing results. The gene ontology terms of DEG were highly enriched in heparin binding (9 genes including COMP, CTGF, and IMPG2), glycosaminoglycan binding (10 genes including PCOLCE, POSTN, and RSPO3), and response to estradiol and ion transport (AREG, RAMP3, SFRP1, and SSTR1). Kyoto encyclopedia of genes and genomes pathway-enrichment analyses of DEG revealed 10 significant pathways (P < 0.05) represented by calcium signaling pathway (7 genes including CACNA1G, PDE1C, PDGFRB, and SLC8A1) and glycosaminoglycan biosynthesis (B3GNT7, CSGALNACT1, GLCE, and ST3GAL1). Protein-protein interaction network of DEG established the connection-regulating epithelial cell or cell-matrix adhesion and migration. The enriched pathways and genes were highly correlated with temporary sperm storage in and possibly sequential sperm release from chicken UVJ overtime after AI. Of these, HIP1, PDE1C, and calcium-related genes were the most interesting candidates associated with sperm storage duration. This report provided a global gene expression profile of the chicken UVJ regarding the capacity of sperm storage overtime after AI. The outcome of this study will contribute to further understanding of the long-term sperm maintenance in avian females and eventually improving the duration of fertile egg performance by selected chicken breeding.
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