Evidence map›Paper›PMID 32036571›Full record

ArticleMolecular biology reports2020

Novel 28 microsatellite loci using high-throughput sequencing for an endangered species on Metasequoia glyptostroboides (Cupressaceae).

Jing-Wen Wang, Tong-Lei Xu, Ghenet Weldegebriel Sereke, Rong Wang, Yuan-Yuan Li

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Article in Molecular biology reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Jing-Wen WangZhejiang Tiantong Forest Ecosystem National Observation and Research Station, Shanghai Key Laboratory for Urban Ecology and Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, China.
Tong-Lei XuZhejiang Tiantong Forest Ecosystem National Observation and Research Station, Shanghai Key Laboratory for Urban Ecology and Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, China.
Ghenet Weldegebriel SerekeZhejiang Tiantong Forest Ecosystem National Observation and Research Station, Shanghai Key Laboratory for Urban Ecology and Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, China.
Rong WangZhejiang Tiantong Forest Ecosystem National Observation and Research Station, Shanghai Key Laboratory for Urban Ecology and Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, China.
Yuan-Yuan LiZhejiang Tiantong Forest Ecosystem National Observation and Research Station, Shanghai Key Laboratory for Urban Ecology and Restoration, School of Ecological and Environmental Sciences, East China Normal University, Shanghai, 200241, China. yyli@des.ecnu.edu.cn.ORCID http://orcid.org/0000-0003-4711-4294

Funding

National Key Research and Development Program 2016YFC0503102National Natural Science Foundation of China 31670540
6 · The paper itself

Abstract

Metasequoia glyptostroboides is a living fossil and an endangered species listed in the International Union for Conservation of Nature (IUCN). Distinguishing the genotypes of all wild individuals of M. glyptostroboides is important to delimit management units and key germplasm resources. We characterized 28 novel polymorphic microsatellite loci using a streptavidin-biotin microsatellite-enriched library and Illumina high-throughput sequencing. Characteristics of each locus were tested using 140 individuals collected from five natural populations of M. glyptostroboides. The number of alleles per locus ranged from 3 to 20, with a mean number of about 8 alleles. The observed and expected heterozygosities in each population ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8958, respectively. Four to nine loci were cross-amplified successfully in seven species of Cupressaceae. The novel SSR markers will provide a toolkit for DNA identification of all of the extant wild individuals guiding further conservation efforts of M. glyptostroboides.

Indexed as

AllelesChinaConservation of Natural ResourcesCupressaceaeDNA PrimersEndangered SpeciesGenomicsGenotypeHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsPolymorphism, GeneticTreesDNA PrimersCupressaceaeGenetic structureGermplasm resourcesIllumina sequencingMetasequoia glyptostroboides

Identifiers

PMID32036571

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