ArticlePlanta2020
Anthocyanin regulatory and structural genes associated with violet flower color of Matthiola incana.
Article in Planta, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 19 citations in OpenAlex.
- Engineering plant architecture in the ornamental species Eustoma grandiflorum by knockout of strigolactone biosynthesis.Plant cell reports · 2026Article
- Largest genome assembly in Brassicaceae: retrotransposon-driven genome expansion and karyotype evolution in Matthiola incana.Plant biotechnology journal · 2025Article
- Genome-wide transcriptome analysis reveal the molecular mechanism for triggering the formation of purple leaves in rice mutantsFrontiers in plant science · 2025Article
- Genome-wide identification and expression profiling of bHLH transcription factors associated with ferulic acid biosynthesis inFrontiers in plant science · 2025Article
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- The transcription factor IbNAC29 positively regulates the carotenoid accumulation in sweet potato.Horticulture research · 2023Article
- Genome-Wide Identification and Characterization ofInternational journal of molecular sciences · 2023Article
- Establishment of an efficient transformation method of garden stock (Plant biotechnology (Tokyo, Japan) · 2022Article
- Comparative transcriptome analyses reveal genes related to pigmentation in the petals of a flower color variation cultivar of Rhododendron obtusum.Molecular biology reports · 2022Article
- DhMYB2 and DhbHLH1 regulates anthocyanin accumulationFrontiers in plant science · 2022Article
- AcMYB1 Interacts With AcbHLH1 to Regulate Anthocyanin Biosynthesis inFrontiers in plant science · 2022Article
- A light-responsive transcription factor SmMYB35 enhances anthocyanin biosynthesis in eggplant (Solanum melongena L.).Planta · 2021Article
- Composition of Flavonoids in the Petals ofFrontiers in plant science · 2021Article
- Dynamic Changes of the Anthocyanin Biosynthesis Mechanism During the Development of Heading Chinese Cabbage (Frontiers in plant science · 2020Article
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Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
Abstract
MAIN
conclusionMiMYB1 and MibHLH2 play key roles in anthocyanin biosynthesis in Matthiola incana flowers. We established a transient expression system using Turnip mosaic virus vector in M. incana. Garden stock (Matthiola incana (L.) R. Br.) is a popular flowering plant observed from winter to spring in Japan. Here we observed that anthocyanin accumulation in 'Vintage Lavender' increased with flower development, whereas flavonol accumulation remained constant throughout flower development. We obtained five transcription factor genes, MiMYB1, MibHLH1, MibHLH2, MiWDR1, and MiWDR2, from M. incana floral cDNA contigs. Yeast two-hybrid analyses revealed that MiMYB1 interacted with MibHLH1, MibHLH2, and MiWDR1, but MiWDR2 did not interact with any transcription factor. Expression levels of MiMYB1 and MibHLH2 increased in petals during floral bud development. Their expression profiles correlated well with the temporal profiles of MiF3'H, MiDFR, MiANS, and Mi3GT transcripts and anthocyanin accumulation profile. On the other hand, MibHLH1 was expressed weakly in all organs of 'Vintage Lavender'. However, high expression levels of MibHLH1 were detected in petals of other cultivars with higher levels of anthocyanin accumulation than 'Vintage Lavender'. MiWDR1 and MiWDR2 maintained constant expression levels in petals during flower development and vegetative organs. Transient MiMYB1 expression in 1-month-old M. incana seedlings using a Turnip mosaic virus vector activated transcription of the endogenous anthocyanin biosynthetic genes MiF3'H, MiDFR, and MiANS and induced ectopic anthocyanin accumulation in leaves. Therefore, MiMYB1 possibly interacts with MibHLH2 and MiWDR1, and this trimeric protein complex activates the transcription of anthocyanin biosynthetic genes in M. incana flowers. Moreover, MibHLH1 acts as an enhancer of anthocyanin biosynthesis with the MiMYB1-MibHLH2-MiWDR1 complex. This study revealed the molecular mechanism involved in the regulation of anthocyanin accumulation levels in M. incana flowers.
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