ArticleScientific reports2020
Fluopack screening platform for unbiased cellular phenotype profiling.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
7 citing papers in PubMed, 15 citations in OpenAlex.
- TMEM41B Is an Interferon-Stimulated Gene That Promotes Pseudorabies Virus Replication.Journal of virology · 2023Article
- Regulation of ER-derived membrane dynamics by the DedA domain-containing proteins VMP1 and TMEM41B.EMBO reports · 2022Review
- Genome-scale CRISPR screen identifies TMEM41B as a multi-function host factor required for coronavirus replication.PLoS pathogens · 2021Article
- TMEM41B is a host factor required for the replication of diverse coronaviruses including SARS-CoV-2.PLoS pathogens · 2021Article
- Image-based profiling for drug discovery: due for a machine-learning upgrade?Nature reviews. Drug discovery · 2021Review
- TMEM41B Is a Pan-flavivirus Host Factor.Cell · 2021Article
- TMEM41B is a pan-flavivirus host factor.bioRxiv : the preprint server for biology · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene and compound functions are often interrogated by perturbation. However, we have limited methods to capture associated phenotypes in an unbiased and holistic manner. Here, we describe Fluopack screening as a novel platform enabling the profiling of subcellular phenotypes associated with perturbation. Our approach leverages imaging of a panel of fluorescent chemical probes to survey cellular processes in an unbiased and high throughput fashion. Segmentation-free, whole image analysis applied to Fluopack images identifies probes revealing distinct phenotypes upon perturbation, thereby informing on the function and mechanism of action of perturbagens. This chemical biology approach allows to interrogate phenotypes that tend to be overlooked by other methods, such as lipid trafficking and ion concentration inside the cell. Fluopack screening is a powerful approach to study orphan protein function, as exemplified by the characterization of TMEM41B as novel regulator of lipid mobilization.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.