ArticleInternational journal of molecular sciences2020
Expanding the CRISPR Toolbox in
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 56 citations in OpenAlex.
- Validation of a Modular Base Editing Strategy, Free-BE, inPlant direct · 2026Article
- Transgene-free genome editing in plants.aBIOTECH · 2026Review
- Genome Editing Approaches in Flax (International journal of molecular sciences · 2026Review
- Review
- From Lab to Field: CRISPRing Major CultivatedInternational journal of molecular sciences · 2026Review
- Molecular breeding of tomato: Advances and challenges.Journal of integrative plant biology · 2025Review
- Recent Advances in Studying the Regulation of Fruit Ripening in Tomato Using Genetic Engineering Approaches.International journal of molecular sciences · 2024Review
- Cytosine base editors optimized for genome editing in potato protoplasts.Frontiers in genome editing · 2023Article
- Review
- Biotechnological Advances to Improve Abiotic Stress Tolerance in Crops.International journal of molecular sciences · 2022Review
- Exploring C-to-G and A-to-Y Base Editing in Rice by Using New Vector Tools.International journal of molecular sciences · 2022Article
- CRISPR-BETS: a base-editing design tool for generating stop codons.Plant biotechnology journal · 2022Article
- Base Editors for Citrus Gene Editing.Frontiers in genome editing · 2022Article
- CRISPR/Cas Genome Editing in Potato: Current Status and Future Perspectives.Frontiers in genetics · 2022Article
- CRISPR-Cas9 Targeting of theFrontiers in microbiology · 2022Article
- CRISPR/Cas- and Topical RNAi-Based Technologies for Crop Management and Improvement: Reviewing the Risk Assessment and Challenges Towards a More Sustainable Agriculture.Frontiers in bioengineering and biotechnology · 2022Review
- CRISPR/Cas9-mediated fine-tuning of miRNA expression in tetraploid potato.Horticulture research · 2022Article
- Genome editing of polyploid crops: prospects, achievements and bottlenecks.Transgenic research · 2021Review
- Novel CRISPR/Cas applications in plants: from prime editing to chromosome engineering.Transgenic research · 2021Review
- Genome editing in fruit, ornamental, and industrial crops.Transgenic research · 2021Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Genome editing has become a major tool for both functional studies and plant breeding in several species. Besides generating knockouts through the classical CRISPR-Cas9 system, recent development of CRISPR base editing holds great and exciting opportunities for the production of gain-of-function mutants. The PAM requirement is a strong limitation for CRISPR technologies such as base editing, because the base substitution mainly occurs in a small edition window. As precise single amino-acid substitution can be responsible for functions associated to some domains or agronomic traits, development of Cas9 variants with relaxed PAM recognition is of upmost importance for gene function analysis and plant breeding. Recently, the SpCas9-NG variant that recognizes the NGN PAM has been successfully tested in plants, mainly in monocotyledon species. In this work, we studied the efficiency of SpCas9-NG in the model moss
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.