ArticleJournal of cellular and molecular medicine2020
First-time characterization of viable but non-culturable Proteus mirabilis: Induction and resuscitation.
Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
18 citing papers in PubMed, 32 citations in OpenAlex.
- From Dormancy to Viability: The Resuscitation Processes of Viable but Non-Culturable Bacteria-A Systematic Review.Microorganisms · 2026Review
- Current status of detection strategies for pathogenic Escherichia coli: a brief review and summary.Analytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2025Review
- In silico investigation and expression analysis of two-component regulatory systems in Proteus mirabilis.Molecular biology reports · 2025Article
- Significance of Viable But Non-culturable (VBNC) State in Vibrios and Other Pathogenic Bacteria: Induction, Detection and the Role of Resuscitation Promoting Factors (Rpf).Current microbiology · 2024Review
- BD Vacutainer™ Urine Culture & Sensitivity Preservative PLUS Plastic Tubes Minimize the Harmful Impact of Stressors Dependent on Temperature and Time Storage in Uropathogenic Bacteria.Journal of clinical medicine · 2024Article
- Potential antivirulence activity of sub-inhibitory concentrations of ciprofloxacin against Proteus mirabilis isolates: an in-vitro and in-vivo study.Annals of clinical microbiology and antimicrobials · 2024Article
- Uncultured Microorganisms and Their Functions in the Fermentation Systems of Traditional Chinese Fermented Foods.Foods (Basel, Switzerland) · 2023Review
- Quantitative Proteomic Analysis on the Slightly Acidic Electrolyzed Water Triggered Viable but Non-CulturableInternational journal of molecular sciences · 2023Article
- Current Perspectives on Viable but Non-Culturable Foodborne Pathogenic Bacteria: A Review.Foods (Basel, Switzerland) · 2023Review
- Transcriptomic analysis ofFrontiers in microbiology · 2023Article
- Host defense against the infection ofFrontiers in cellular and infection microbiology · 2022Review
- Silicone Foley catheters impregnated with microbial indole derivatives inhibit crystalline biofilm formation byFrontiers in cellular and infection microbiology · 2022Article
- Case Report: An Ulceration With a Stalactite Appearance on the Index Finger.Frontiers in medicine · 2022Article
- The Relationship between Antibiotic Susceptibility and pH in the Case of Uropathogenic Bacteria.Antibiotics (Basel, Switzerland) · 2021Article
- Induction and Resuscitation of Viable but NonculturableMicroorganisms · 2021Article
- Indole Derivatives Obtained from EgyptianAntibiotics (Basel, Switzerland) · 2021Article
- Nosocomial Outbreak of Carbapenemase-ProducingFrontiers in microbiology · 2021Article
- First-time characterization of viable but non-culturable Proteus mirabilis: Induction and resuscitation.Journal of cellular and molecular medicine · 2020Article
Corrections and comments
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Authors and funding
4 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenic bacteria can enter into a viable but non-culturable (VBNC) state under unfavourable conditions. Proteus mirabilis is responsible for dire clinical consequences including septicaemia, urinary tract infections and pneumonia, but is not a species previously known to enter VBNC state. We suggested that stress-induced P. mirabilis can enter a VBNC state in which it retains virulence. P. mirabilis isolates were incubated in extreme osmotic pressure, starvation, low temperature and low pH to induce a VBNC state. Resuscitation was induced by temperature upshift and inoculation in tryptone soy broth with Tween 20 and brain heart infusion broth. Cellular ultrastructure and gene expression were examined using transmission electron microscopy (TEM) and quantitative real-time polymerase chain reaction (qPCR), respectively. High osmotic pressure and low acidity caused rapid entry into VBNC state. Temperature upshift caused the highest percentage of resuscitation (93%) under different induction conditions. In the VBNC state, cells showed aberrant and dwarf morphology, virulence genes and stress response genes (envZ and rpoS) were expressed (levels varied depending on strain and inducing factors). This is the first-time characterization of VBNC P. mirabilis. The ability of P. mirabilis pathogenic strains to enter a stress-induced VBNC state can be a serious public health threat.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.