ArticleOdontology2020
The beneficial effect of cold atmospheric plasma on parameters of molecules and cell function involved in wound healing in human osteoblast-like cells in vitro.
Article in Odontology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Non-Invasive Physical Plasma Regulates COX-2 via EP2/EP4 Expression in Periodontal Ligament Cells Under Inflammatory Conditions.International journal of molecular sciences · 2026Article
- Review
- The effect of cold atmospheric plasma on inflammatory and joint destructive factors in osteoarthritic fibroblast-like synoviocytes.Clinical rheumatology · 2025Article
- Advancing chronic and acute wound healing with cold atmospheric plasma: cellular and molecular mechanisms, benefits, risks, and future directions.Frontiers in medicine · 2025Review
- Decontaminative Properties of Cold Atmospheric Plasma Treatment on Collagen Membranes Used for Guided Bone Regeneration.Journal of functional biomaterials · 2023Article
- Low temperature plasma suppresses proliferation, invasion, migration and survival of SK-BR-3 breast cancer cells.Molecular biology reports · 2023Article
- Inhibitory Effects of Cold Atmospheric Plasma on Inflammation and Tumor-Like Feature of Fibroblast-Like Synoviocytes from Patients with Rheumatoid Arthritis.Inflammation · 2022Article
- Modulation of Inflammatory Responses by a Non-Invasive Physical Plasma Jet during Gingival Wound Healing.Cells · 2022Article
- Cold Atmospheric Plasma Targeting Hematological Malignancies: Potentials and Problems of Clinical Translation.Antioxidants (Basel, Switzerland) · 2022Review
- Article
- Applications of Plasma-Activated Water in Dentistry: A Review.International journal of molecular sciences · 2022Review
- Influence of cold atmospheric plasma on dental implant materials - an in vitro analysis.Clinical oral investigations · 2022Article
- Effect of Cold Atmospheric Plasma (CAP) on Osteogenic Differentiation Potential of Human Osteoblasts.International journal of molecular sciences · 2022Article
- Biocompatible Gas Plasma Treatment Affects Secretion Profiles but Not Osteogenic Differentiation in Patient-Derived Mesenchymal Stromal Cells.International journal of molecular sciences · 2022Article
- Evaluation of the protective effects of non-thermal atmospheric plasma on alveolar bone loss in experimental periodontitis.Clinical oral investigations · 2021Article
- Cold Atmospheric Plasma Jet as a Possible Adjuvant Therapy for Periodontal Disease.Molecules (Basel, Switzerland) · 2021Article
- Influences of cold atmospheric plasma on apoptosis related molecules in osteoblast-like cells in vitro.Head & face medicine · 2021Article
- Synergistic effect of cold atmospheric pressure plasma and free or liposomal doxorubicin on melanoma cells.Scientific reports · 2021Article
- Cold Atmospheric Plasma Promotes Regeneration-Associated Cell Functions of Murine Cementoblasts In Vitro.International journal of molecular sciences · 2021Article
- Inhibiting proliferation and metastasis of osteosarcoma cells by downregulation of long non-coding RNA colon cancer-associated transcript 2 targeting microRNA-143.Oncology letters · 2021Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The aim of this study was to analyse the effect of cold atmospheric plasma (CAP) on human osteoblast-like cells in vitro. Additionally, underlying intracellular mechanisms were to be studied. Human osteoblast-like (MG63) cells were exposed to CAP for 60 s. The effects of CAP on key molecules essential for the wound healing response were studied using real-time PCR, ELISA and immunocytochemistry. For studying intracellular signalling pathways, MAP kinase MEK 1/2 was blocked. Cell viability was analysed by an XTT assay and with an EVE automated cell counter. Cell migration was examined by an in vitro wound healing assay.CAP exposition on osteoblast-like cells caused a significant upregulation of interleukin (IL)-1β, IL-6, IL-8, tumor necrosis factor (TNF)α, cyclooxygenase (COX)2, collagen (COL) 1α, matrix metalloproteinase (MMP)1, Ki67, proliferating-cell-nuclear-antigen (PCNA) and chemokine ligand (CCL)2 mRNA expression at 1 day. Interestingly, after blocking of MAP kinase, CAP-induced upregulation of Ki67 was inhibited by 57%. Moreover, CAP treatment improved significantly osteoblast-like cell viability as compared to untreated cells at 1 day. Beneficial effect of CAP treatment was shown by an in vitro wound healing assay, displaying a significant faster wound closure. Our findings provide evidence that CAP exposure effects gene and protein regulation in human osteoblast-like cells. Furthermore, CAP treatment has a positive impact on wound closure in an in vitro setting and might improve existing concepts of hard tissue regeneration in the future.
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