ArticleCellular and molecular bioengineering2020
Producing Collagen Micro-stripes with Aligned Fibers for Cell Migration Assays.
Article in Cellular and molecular bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.
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Who cites it
10 citing papers in PubMed, 1 synthesis or guideline pooled it, 19 citations in OpenAlex.
- A Systematic Review of Tissue Engineering Scaffold in Tendon Bone HealingFrontiers in bioengineering and biotechnology · 2021Pooled it
- Fabrication of Micropatterns of Aligned Collagen Fibrils.Langmuir : the ACS journal of surfaces and colloids · 2024Article
- Engineering tools for quantifying and manipulating forces in epithelia.Biophysics reviews · 2023Review
- Construction of a Multitubular Perfusable Kidney-on-Chip for the Study of Renal Diseases.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Collagen Remodeling along Cancer Progression Providing a Novel Opportunity for Cancer Diagnosis and Treatment.International journal of molecular sciences · 2022Review
- Collagen polarization promotes epithelial elongation by stimulating locoregional cell proliferation.eLife · 2021Article
- Tumorigenic mesenchymal clusters are less sensitive to moderate osmotic stresses due to low amounts of junctional E-cadherin.Scientific reports · 2021Article
- Engineering fiber anisotropy within natural collagen hydrogels.American journal of physiology. Cell physiology · 2021Review
- Amphiphilic cationic cyclodextrin nanovesicles: a versatile cue for guiding cell adhesion.Nanoscale advances · 2020Article
- Advanced strategies for constructing interfacial tissues of bone and tendon/ligament.Journal of tissue engineeringReview
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionThe orientation of collagen fibers in native tissues plays an important role in cell signaling and mediates the progression of tumor cells in breast cancer by a contact guidance mechanism. Understanding how migration of epithelial cells is directed by the alignment of collagen fibers requires
methodsTo address this issue, we produced micro-stripes with aligned collagen fibers using an easy-to-use and versatile approach based on the aspiration of a collagen solution within a microchannel. Glass coverslips were functionalized with a (3-aminopropyl)triethoxysilane/glutaraldehyde linkage to covalently anchor micro-stripes of aligned collagen fibers, whereas microchannels were functionalized with a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) (PEO-PPO-PEO) nonionic triblock polymer to prevent adhesion of the collagen micro-stripes.
resultsUsing this strategy, microchannels can be peeled off to expose micro-stripes of aligned collagen fibers without affecting their mechanical integrity. We used time-lapse confocal reflection microscopy to characterize the polymerization kinetics of collagen networks for different concentrations and the orientation of collagen fibers as a function of the microchannel width. Our results indicate a non-linear concentration dependence of the area of fluorescence, suggesting that the architecture of collagen networks is sensitive to small changes in concentration. We show the possibility to influence the collagen fibril coverage by adjusting the concentration of the collagen solution.
conclusionWe applied this novel approach to study the migration of epithelial cells, demonstrating that collagen micro-stripes with aligned fibers represent a valuable
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