Evidence map›Paper›PMID 32030106›Full record

ArticleCellular and molecular bioengineering2020

Differential Contributions of Actin and Myosin to the Physical Phenotypes and Invasion of Pancreatic Cancer Cells.

Angelyn V Nguyen, Brittany Trompetto, Xing Haw Marvin Tan, Michael B Scott, Kenneth Hsueh-Heng Hu, Eric Deeds, Manish J Butte, Pei Yu Chiou, Amy C Rowat

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cellular and molecular bioengineering, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 18 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 5 institutions in 1 country.

Angelyn V NguyenDepartment of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.
Brittany TrompettoDepartment of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.
Xing Haw Marvin TanDepartment of Bioengineering, University of California, Los Angeles, USA.
Michael B ScottDepartment of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.
Kenneth Hsueh-Heng HuStanford Biophysics, Stanford University, Stanford, USA.
Eric DeedsDepartment of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.
Manish J ButteDepartment of Pediatrics, University of California, Los Angeles, USA.
Pei Yu ChiouDepartment of Bioengineering, University of California, Los Angeles, USA.
Amy C RowatDepartment of Integrative Biology and Physiology, University of California, 610 Charles E Young Dr. East, Los Angeles, CA 90095 USA.ORCID 0000-0001-5800-5320
University of California, Los Angeles · USCharles R. Drew University of Medicine and Science · USNorthwestern University · USQB3 · USStanford University · US

Funding

Influences of nanomechanical forces on T cellsR01GM110482 · NIGMS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BUTTE, MANISH J · 2014 to 2018
$1.6M
NIGMS NIH HHS R01 GM110482
6 · The paper itself

Abstract

introductionMetastasis is a fundamentally physical process in which cells deform through narrow gaps and generate forces to invade surrounding tissues. While it is commonly thought that increased cell deformability is an advantage for invading cells, we previously found that more invasive pancreatic ductal adenocarcinoma (PDAC) cells are stiffer than less invasive PDAC cells. Here we investigate potential mechanisms of the simultaneous increase in PDAC cell stiffness and invasion, focusing on the contributions of myosin II, Arp2/3, and formins.

methodWe measure cell invasion using a 3D scratch wound invasion assay and cell stiffness using atomic force microscopy (AFM). To determine the effects of actin- and myosin-mediated force generation on cell stiffness and invasion, we treat cells with pharmacologic inhibitors of myosin II (blebbistatin), Arp2/3 (CK-666), and formins (SMIFH2).

resultsWe find that the activity of myosin II, Arp2/3, and formins all contribute to the stiffness of PDAC cells. Interestingly, we find that the invasion of PDAC cell lines is differentially affected when the activity of myosin II, Arp2/3, or formins is inhibited, suggesting that despite having similar tissue origins, different PDAC cell lines may rely on different mechanisms for invasion.

conclusionsThese findings deepen our knowledge of the factors that regulate cancer cell mechanotype and invasion, and incite further studies to develop therapeutics that target multiple mechanisms of invasion for improved clinical benefit.

Indexed as

Arp2/3Cell motilityCell stiffnessCytoskeletonForminsMechanobiologyPancreatic ductal adenocarcinomaTraction forces

Identifiers

PMID32030106
PMCPMC6981337
OpenAlexW2983077790

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.