ArticleScientific reports2020
Rapid and Precise Semi-Automatic Axon Quantification in Human Peripheral Nerves.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
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- Trigeminal-Facial Nerve Anatomical Connections and Their Clinical Value: A Narrative Review.Diagnostics (Basel, Switzerland) · 2026Review
- AxoMetric platform facilitates rapid and unbiased automated quantification of axon regeneration in tissue sections.Cell reports methods · 2026Article
- Distribution of bladder afferent activity across the sacral roots in sheep shows marked individual variation: implications for neuroprosthesis design.Frontiers in neuroscience · 2026Article
- Efficient and Rapid Histomorphometry of Regenerating Peripheral Nerve.Muscle & nerve · 2025Article
- Brachialis to Anterior Interosseous Nerve Transfer: Comprehensive Anatomic Rationale.Operative neurosurgery (Hagerstown, Md.) · 2025Article
- Histomorphometry of the Sural Nerve for Use as a CFNG in Facial Reanimation Procedures.Journal of clinical medicine · 2023Article
- Selective ansa cervicalis nerve transfer to the marginal mandibular nerve for lower lip reanimation: An anatomical study in cadavers and a case report.Microsurgery · 2023Article
- Axonal mapping of the motor cranial nerves.Frontiers in neuroanatomy · 2023Article
- A Ready-to-Use Grading Tool for Facial Palsy Examiners-Automated Grading System in Facial Palsy Patients Made Easy.Journal of personalized medicine · 2022Article
- Reply to Nduka et al. Comment on "Kehrer et al. Using High-Resolution Ultrasound to Assess Post-Facial Paralysis Synkinesis-Machine Settings and Technical Aspects for Facial Surgeons.Diagnostics (Basel, Switzerland) · 2022Article
- Using High-Resolution Ultrasound to Assess Post-Facial Paralysis Synkinesis-Machine Settings and Technical Aspects for Facial Surgeons.Diagnostics (Basel, Switzerland) · 2022Article
- A revisit to staining reagents for neuronal tissues.Annals of eye science · 2022Article
- Validation of the Use of Automated and Manual Quantitative Analysis of Corneal Nerve Plexus Following Refractive Surgery.Diagnostics (Basel, Switzerland) · 2020Article
- Neurodegeneration of the mastoid segment of the human facial nerve associated with senescence.Brazilian journal of otorhinolaryngologyArticle
Corrections and comments
- Erratum issued
Authors and funding
10 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We developed a time-efficient semi-automated axon quantification method using freeware in human cranial nerve sections stained with paraphenylenediamine (PPD). It was used to analyze a total of 1238 facial and masseteric nerve biopsies. The technique was validated by comparing manual and semi-automated quantification of 129 (10.4%) randomly selected biopsies. The software-based method demonstrated a sensitivity of 94% and a specificity of 87%. Semi-automatic axon counting was significantly faster (p < 0.001) than manual counting. It took 1 hour and 47 minutes for all 129 biopsies (averaging 50 sec per biopsy, 0.04 seconds per axon). The counting process is automatic and does not need to be supervised. Manual counting took 21 hours and 6 minutes in total (average 9 minutes and 49 seconds per biopsy, 0.52 seconds per axon). Our method showed a linear correlation to the manual counts (R = 0.944 Spearman rho). Attempts have been made by several research groups to automate axonal load quantification. These methods often require specific hard- and software and are therefore only accessible to a few specialized laboratories. Our semi-automated axon quantification is precise, reliable and time-sparing using publicly available software and should be useful for an effective axon quantification in various human peripheral nerves.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.