ArticleCommunications biology2020
Perpendicular alignment of lymphatic endothelial cells in response to spatial gradients in wall shear stress.
Article in Communications biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Differential biomarker expression of blood and lymphatic vasculature in multi-organ-chips.Scientific reports · 2025Article
- VE-PTP controls a fluid shear stress set point that governs cell morphological responses through Tie-2.Frontiers in cell and developmental biology · 2025Article
- Fluid-Dynamic Modeling of Flow in Embryonic Tissue Indicates That Lymphatic Valve Location Is Not Consistently Determined by the Local Fluid Shear or Its Gradient.Microcirculation (New York, N.Y. : 1994) · 2024Article
- Targeted Rapamycin Delivery via Magnetic Nanoparticles to Address Stenosis in a 3D Bioprinted in Vitro Model of Pulmonary Veins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Response of lymphatic endothelial cells to combined spatial and temporal variations in fluid flow.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2023Article
- A 3D Human Lymphatic Vessel-on-Chip Reveals the Roles of Interstitial Flow and VEGF-A/C for Lymphatic Sprouting and Discontinuous Junction Formation.Cellular and molecular bioengineering · 2023Article
- Modeling lymphangiogenesis: Pairing in vitro and in vivo metrics.Microcirculation (New York, N.Y. : 1994) · 2023Review
- A Review of Functional Analysis of Endothelial Cells in Flow Chambers.Journal of functional biomaterials · 2022Review
- Effect of Human Synovial Fluid From Osteoarthritis Patients and Healthy Individuals on Lymphatic Contractile Activity.Journal of biomechanical engineering · 2022Article
- Intracellular calcium dynamics of lymphatic endothelial and muscle cells co-cultured in a Lymphangion-Chip under pulsatile flow.The Analyst · 2022Article
- A miniaturized 3D printed pressure regulator (µPR) for microfluidic cell culture applications.Scientific reports · 2022Article
- Bio-printing of aligned GelMa-based cell-laden structure for muscle tissue regeneration.Bioactive materials · 2022Article
- Lymphangion-chip: a microphysiological system which supports co-culture and bidirectional signaling of lymphatic endothelial and muscle cells.Lab on a chip · 2021Article
- Aortic valve cell microenvironment: Considerations for developing a valve-on-chip.Biophysics reviews · 2021Review
- A 3D Bioprinted In Vitro Model of Pulmonary Artery Atresia to Evaluate Endothelial Cell Response to Microenvironment.Advanced healthcare materials · 2021Article
- Mutations in EPHB4 cause human venous valve aplasia.JCI insight · 2021Article
- Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology.Communications biology · 2021Review
- A high-resolution real-time quantification of astrocyte cytokine secretion under shear stress for investigating hydrocephalus shunt failure.Communications biology · 2021Article
- Endothelial cells do not align with the mean wall shear stress vector.Journal of the Royal Society, Interface · 2021Article
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Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
One-way valves in the lymphatic system form from lymphatic endothelial cells (LECs) during embryonic development and are required for efficient tissue drainage. Although fluid flow is thought to guide both valve formation and maintenance, how this occurs at a mechanistic level remains incompletely understood. We built microfluidic devices that reproduce critical aspects of the fluid flow patterns found at sites of valvulogenesis. Using these devices, we observed that LECs replicated aspects of the early steps in valvulogenesis: cells oriented perpendicular to flow in the region of maximum wall shear stress (WSS) and exhibited enhanced nuclear localization of FOXC2, a transcription factor required for valvulogenesis. Further experiments revealed that the cell surface protein E-selectin was required for both of these responses. Our observations suggest that spatial gradients in WSS help to demarcate the locations of valve formation, and implicate E-selectin as a component of a mechanosensory process for detecting WSS gradients.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.