ArticleScientific reports2020
On-site single pollen metabolomics reveals varietal differences in phosphatidylinositol synthesis under heat stress conditions in rice.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 36 citations in OpenAlex.
- Untargeted metabolomics suggests a trade-off between phenolamides and flavonoids in the pollen of Petunia hybrida with contrasting flower colours.BMC plant biology · 2026Article
- Anther and pollen thermotolerance: molecular mechanisms and implications for plant reproduction under heat stress.Planta · 2026Review
- 'Roly-poly toy' motion during pollen exudation promotes rapid pollen adhesion in rice.Communications biology · 2025Article
- Decrypting molecular mechanism of heat stress tolerance in rice to tackle climate change challenges through recent approaches.Frontiers in plant science · 2025Review
- Heat stress at the bicellular stage inhibits sperm cell development and transport into pollen tubes.Plant physiology · 2024Article
- Advances in single-cell metabolomics to unravel cellular heterogeneity in plant biology.Plant physiology · 2023Review
- Recent Molecular Aspects and Integrated Omics Strategies for Understanding the Abiotic Stress Tolerance of Rice.Plants (Basel, Switzerland) · 2023Review
- Dynamics and stabilization mechanism of mitochondrial cristae morphofunction associated with turgor-driven cardiolipin biosynthesis under salt stress conditions.Scientific reports · 2022Article
- Metabolomics: a systems biology approach for enhancing heat stress tolerance in plants.Plant cell reports · 2022Review
- Metabolomics and Transcriptomics Analysis of Pollen Germination Response to Low-Temperature in Pitaya (Frontiers in plant science · 2022Article
- Direct evidence for dynamics of cell heterogeneity in watercored apples: turgor-associated metabolic modifications and within-fruit water potential gradient unveiled by single-cell analyses.Horticulture research · 2021Article
- Applications of ambient ionization mass spectrometry in 2020: An annual review.Analytical science advances · 2021Review
- High temperature-mediated disturbance of carbohydrate metabolism and gene expressional regulation in rice: a review.Plant signaling & behavior · 2021Review
- Endosperm cell size reduction caused by osmotic adjustment during nighttime warming in rice.Scientific reports · 2021Article
- Transcriptome Co-expression Network Analysis Identifies Key Genes Regulating Conchosporangia Maturation ofFrontiers in genetics · 2021Article
- Proline Accumulation in Pollen Grains as Potential Target for Improved Yield Stability Under Salt Stress.Frontiers in plant science · 2020Article
Corrections and comments
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Authors and funding
10 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although a loss of healthy pollen grains induced by metabolic heat responses has been indicated to be a major cause of heat-induced spikelet sterility under global climate change, to date detailed information at pollen level has been lacking due to the technical limitations. In this study, we used picolitre pressure-probe-electrospray-ionization mass spectrometry (picoPPESI-MS) to directly determine the metabolites in heat-treated single mature pollen grains in two cultivars, heat-tolerant cultivar, N22 and heat-sensitive cultivar, Koshihikari. Heat-induced spikelet fertility in N22 and Koshihikari was 90.0% and 46.8%, respectively. While no treatment difference in in vitro pollen viability was observed in each cultivar, contrasting varietal differences in phosphatidylinositol (PI)(34:3) have been detected in mature pollen, together with other 106 metabolites. Greater PI content was detected in N22 pollen regardless of the treatment, but not for Koshihikari pollen. In contrast, there was little detection for phosphoinositide in the single mature pollen grains in both cultivars. Our findings indicate that picoPPESI-MS analysis can efficiently identify the metabolites in intact single pollen. Since PI is a precursor of phosphoinositide that induces multiple signaling for pollen germination and tube growth, the active synthesis of PI(34:3) prior to germination may be closely associated with sustaining spikelet fertility even at high temperatures.
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