ArticleScientific reports2020
Automated high-throughput heartbeat quantification in medaka and zebrafish embryos under physiological conditions.
Article in Scientific reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- An Anesthesia-Free Pharmacological Assay for Zebrafish Heart Failure Models Using a Microfluidic Platform.Biomedicines · 2026Article
- Heart rate control by listening to light.Nature communications · 2026Article
- A genetic model for development, physiology and behavior of catecholamine-deficient zebrafish larvae.Communications biology · 2026Article
- Zebrafish model for studying vascular development and vascular-related disease.Frontiers in cell and developmental biology · 2026Review
- Recommendations of the Polish Zebrafish Society on the use of the zebrafish (Acta biochimica Polonica · 2026Review
- Exploring universal segmentation models for automatic quantification of cardiac functional parameters from zebrafish heartbeat videos.Medical & biological engineering & computing · 2026Article
- Automated measurement of cardiomyocyte monolayer contraction using the Exeter Multiscope.Biomedical optics express · 2025Article
- Current status and challenges of multi-omics research using animal models of atherosclerosis.Journal of molecular and cellular cardiology plus · 2025Review
- Effects of Underwater Noise Exposure on Early Development in Zebrafish.Animals : an open access journal from MDPI · 2025Article
- Natural genetic variation quantitatively regulates heart rate and dimension.Nature communications · 2025Article
- Polystyrene nanoplastics impact the bioenergetics of developing zebrafish and limit molecular and physiological adaptive responses to acute temperature stress.The Science of the total environment · 2025Article
- FEHAT: efficient, large scale and automated heartbeat detection in Medaka fish embryos.Bioinformatics (Oxford, England) · 2024Article
- Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis.eLife · 2024Article
- Diving into drug-screening: zebrafish embryos as an in vivo platform for antimicrobial drug discovery and assessment.FEMS microbiology reviews · 2024Review
- Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis.bioRxiv : the preprint server for biology · 2024Article
- Plxnd1-mediated mechanosensing of blood flow controls the caliber of the Dorsal Aorta via the transcription factor Klf2.bioRxiv : the preprint server for biology · 2024Article
- Hemodynamics During Development and Postnatal Life.Advances in experimental medicine and biology · 2024Review
- Hexavalent Chromium Inhibited Zebrafish Embryo Development by Altering Apoptosis- and Antioxidant-Related Genes.Current issues in molecular biology · 2023Article
- CRISPR-based knockout and base editing confirm the role of MYRF in heart development and congenital heart disease.Disease models & mechanisms · 2023Article
- Transcriptome Analysis of Thiram-Treated Zebrafish (Biology · 2023Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Accurate quantification of heartbeats in fish models is an important readout to study cardiovascular biology, disease states and pharmacology. However, dependence on anaesthesia, laborious sample orientation or requirement for fluorescent reporters have hampered the use of high-throughput heartbeat analysis. To overcome these limitations, we established an efficient screening assay employing automated label-free heart rate determination of randomly oriented, non-anesthetized medaka (Oryzias latipes) and zebrafish (Danio rerio) embryos in microtiter plates. Automatically acquired bright-field data feeds into an easy-to-use HeartBeat software with graphical user interface for automated quantification of heart rate and rhythm. Sensitivity of the assay was demonstrated by profiling heart rates during entire embryonic development. Our analysis revealed rapid adaption of heart rates to temperature changes, which has implications for standardization of experimental layout. The assay allows scoring of multiple embryos per well enabling a throughput of >500 embryos per 96-well plate. In a proof of principle screen for compound testing, we captured concentration-dependent effects of nifedipine and terfenadine over time. Our novel assay permits large-scale applications ranging from phenotypic screening, interrogation of gene functions to cardiovascular drug development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.