ArticlePoultry science2020
Molecular detection and characterization of Mycoplasma gallisepticum and Mycoplasma synoviae strains in backyard poultry in Italy.
Article in Poultry science, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 34 citations in OpenAlex.
- Hydroxytyrosol Restrains NLRP3 Inflammasome Activation and Pyroptosis Through the TLR4/NF-κB Pathway to Prevent Mycoplasma gallisepticum-Inflammation.Poultry science · 2026Article
- Development and validation of a rapid triple-target gold nanoparticle lateral flow immunoassay for detection of three avian mycoplasmas immunochromatographic strip test for avian mycoplasma detection.BMC microbiology · 2026Article
- Molecular typing ofApplied and environmental microbiology · 2026Article
- Pathogenic mechanisms and vaccine development forFrontiers in microbiology · 2025Review
- Molecular detection and genetic characterization of Mycoplasma gallisepticum and Mycoplasma synoviae in selected chicken breeds in South Africa.BMC infectious diseases · 2024Article
- Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco.Veterinary world · 2023Article
- Drug sensitivity and genome-wide analysis of two strains ofFrontiers in microbiology · 2023Article
- Longitudinal Survey on aMPV Circulation in French Broiler Flocks following Different Vaccination Strategies.Animals : an open access journal from MDPI · 2022Article
- Rapid and visual detection of Mycoplasma synoviae by recombinase-aided amplification assay combined with a lateral flow dipstick.Poultry science · 2022Article
- Targeted sequencing analysis of Mycoplasma gallisepticum isolates in chicken layer and breeder flocks in Thailand.Scientific reports · 2022Article
- First Molecular Survey to DetectAnimals : an open access journal from MDPI · 2022Article
- New Insights into the Host-Pathogen Interaction ofMicroorganisms · 2021Article
- Backyard poultry cases in UK small animal practices: Demographics, health conditions and pharmaceutical prescriptions.The Veterinary record · 2021Article
- Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl.Animals : an open access journal from MDPI · 2021Article
- Seroprevalence and prevalence of Mycoplasma synoviae in laying hens and broiler breeders in Spain.Poultry science · 2021Article
- Concurrent infection of Avibacterium paragallinarum and fowl adenovirus in layer chickens.Poultry science · 2020Article
- Molecular Differentiation ofVaccines · 2020Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) represent the most important avian Mycoplasma species in the poultry industry, causing considerable economic losses. In Italy, the presence of MG or MS has been investigated especially in commercial poultry farms. To our knowledge, no systematic investigations on MG or MS presence using highly specific diagnostic assays have been performed in backyard poultry. The aim of this study was to detect and molecularly characterize MG and MS strains in 11 backyard poultry flocks located in different regions of Italy. Tracheal swabs were collected and DNA was extracted. For MS, a PCR targeting a vlhA gene fragment was performed, and typing and subtyping was attempted. The presence of MG was investigated by a screening PCR, then MG typing by gene-targeted sequencing (GTS). All the amplicons were sequenced, then MG and MS dendrograms were constructed. All the flocks examined resulted Mycoplasma positive: 5 out of 11 (45.45%) were MG and MS positive, 3 (27.27%) were MG positive, and the remaining 3 (27.27%) were MS positive. The MS detections were assigned to types C, D, and F. All strains of type D belonged to subtype D1 and 2 unknown subtypes were identified. A MS sequence showed peculiar characteristics, which did not allow assignment to a known MS type or subtype. MG GTS analysis identified 6 MG strains belonging to 5 subclusters circulating in Italian backyards chicken flocks. The results of this study provide evidence of a risk for commercial poultry farms, especially in areas where backyard and commercial farms are close, suggesting the implementation of biosecurity measures.
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