ArticleJournal of dental research2020
VE-Cadherin and Anastomosis of Blood Vessels Formed by Dental Stem Cells.
Article in Journal of dental research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Effectiveness of root canal therapy in auto-transplanted third molars: a systematic review, meta-analysis and case series report.Clinical oral investigations · 2025Pooled it
- Article
- Long-Term Biobanked Dental Pulp Stem Cells Retain Angiogenic Potential for Vascularised Tissue Engineering-Laboratory Investigation.International endodontic journal · 2026Article
- Meningeal lymphatic drainage: novel insights into central nervous system disease.Signal transduction and targeted therapy · 2025Review
- Guidance for evaluating biomaterials' properties and biological potential for dental pulp tissue engineering and regeneration research.Dental materials : official publication of the Academy of Dental Materials · 2025Review
- DMP1-mediated transformation of DPSCs to CD31Frontiers in cell and developmental biology · 2025Article
- Multiscale Imaging to Monitor Functional SHED-Supported Engineered Vessels.Journal of dental research · 2024Article
- Dental pulp regeneration strategies: A review of status quo and recent advances.Bioactive materials · 2024Review
- Neovascularization by DPSC-ECs in a Tube Model for Pulp Regeneration Study.Journal of dental research · 2024Article
- Dentin matrix protein 1 and HUVEC-ECM scaffold promote the differentiation of human dental pulp stem cells into endothelial lineage: implications in regenerative medicine.Frontiers in physiology · 2024Article
- Inactivation of ERK1/2 signaling mediates dysfunction of basal meningeal lymphatic vessels in experimental subdural hematoma.Theranostics · 2024Article
- Stem cells in clinical dentistry.Journal of the American Dental Association (1939) · 2023Review
- Cell-scaffold interactions in tissue engineering for oral and craniofacial reconstruction.Bioactive materials · 2023Article
- Role of Heparan Sulfate in Vasculogenesis of Dental Pulp Stem Cells.Journal of dental research · 2023Article
- Cannabidiol Rescues TNF-α-Inhibited Proliferation, Migration, and Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells.Biomolecules · 2023Article
- Prevascularization techniques for dental pulp regeneration: potential cell sources, intercellular communication and construction strategies.Frontiers in bioengineering and biotechnology · 2023Review
- Decellularized rat submandibular gland as an alternative scaffold for dental pulp regeneration.Frontiers in bioengineering and biotechnology · 2023Article
- Vascularization of a Bone Organoid Using Dental Pulp Stem Cells.Stem cells international · 2023Article
- Chitosan/Alginate Hydrogel Dressing Loaded FGF/VE-Cadherin to Accelerate Full-Thickness Skin Regeneration and More Normal Skin Repairs.International journal of molecular sciences · 2022Article
- PDGF-BB signalingFrontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
It is known that dental pulp stem cells (DPSCs) can be induced to differentiate into vasculogenic endothelial (VE) cells. However, the process that results in sprouting and anastomosis of DPSC-derived vessels remains unclear. Here, we performed studies to understand the mechanisms underpinning the anastomosis of the host vasculature with blood vessels generated by DPSCs (a model for mesenchymal stem cells). VE-cadherin-silenced primary human DPSCs seeded in tooth slice/scaffolds and transplanted into the subcutaneous space of immunodeficient mice generated fewer functional blood vessels (i.e., anastomosed with the host vasculature) than control DPSCs transduced with scrambled sequences. Both VE-cadherin-silenced and mitogen-activated protein kinase kinase 1 (MEK1)-silenced cells showed a decrease in the number of capillary sprouts in vitro. Interestingly, DPSC stably transduced with a VE-cadherin reporter demonstrated that vascular endothelial growth factor (VEGF) induces VE-cadherin expression in sprouting DPSCs undergoing anastomosis, but not in quiescent DPSCs. To begin to understand the mechanisms regulating VE-cadherin, we stably silenced MEK1 and observed that VEGF was no longer able to induce VE-cadherin expression and capillary sprout formation. Notably ERG, a transcriptional factor downstream from MEK/ERK, binds to the promoter region of VE-cadherin (chip assay) and is induced by VEGF in DPSCs. Collectively, these data defined a signaling pathway triggered by VEGF that results in phosphorylation of MEK1/ERK and activation of ERG leading to expression of VE-cadherin, which is required for anastomosis of DPSC-derived blood vessels. In conclusion, these results unveiled a signaling pathway that enables the generation of functional blood vessels upon vasculogenic differentiation of DPSCs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.