ArticleInternational journal of molecular sciences2020
Adipose-Derived Mesenchymal Stem Cell Chondrospheroids Cultured in Hypoxia and a 3D Porous Chitosan/Chitin Nanocrystal Scaffold as a Platform for Cartilage Tissue Engineering.
Article in International journal of molecular sciences, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Chitosan-based Biomaterials in Regenerative Medicine: Optimizing Mesenchymal Stem Cell Viability and Function.Stem cell reviews and reports · 2025Review
- Wet-electrospun porous freeform scaffold enhances colonisation of cells.Materials today. Bio · 2025Article
- Enhancement of Chondrogenic Differentiation in Bone Marrow-Derived Stem Cell Spheroids byJournal of personalized medicine · 2024Article
- The tardigrade-derived mitochondrial abundant heat soluble protein improves adipose-derived stem cell survival against representative stressors.Scientific reports · 2024Article
- Spheroid trilineage differentiation model of primary mesenchymal stem/stromal cells under hypoxia and serum-free culture conditions.Frontiers in bioengineering and biotechnology · 2024Article
- Hypoxia Promotes Cartilage Regeneration in Cell-Seeded 3D-Printed Bioscaffolds Cultured with a Bespoke 3D Culture Device.International journal of molecular sciences · 2023Article
- Double Valorization for a Discard-α-Chitin and Calcium Lactate Production from the CrabMarine drugs · 2022Article
- Mesenchymal stem cells and their microenvironment.Stem cell research & therapy · 2022Review
- Article
- Review
- Evaluation of the Usefulness of Human Adipose-Derived Stem Cell Spheroids Formed Using SphereRingCells · 2022Article
- Directed Regeneration of Osteochondral Tissue by Hierarchical Assembly of Spatially Organized Composite Spheroids.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Articular cartilage and osteochondral tissue engineering techniques: Recent advances and challenges.Bioactive materials · 2021Review
- Reviewing Chitin/Chitosan Nanofibers and Associated Nanocomposites and Their Attained Medical Milestones.Polymers · 2021Review
- Chondrogenic potential of mesenchymal stem cells from horses using a magnetic 3D cell culture system.World journal of stem cells · 2021Article
- Effect of DeterpenatedPolymers · 2021Article
- Article
- Engineered biomaterial strategies for controlling growth factors in tissue engineering.Drug delivery · 2020Review
- Elastin-Collagen Based Hydrogels as Model Scaffolds to Induce Three-Dimensional Adipocyte Culture from Adipose Derived Stem Cells.Bioengineering (Basel, Switzerland) · 2020Article
- Extraction of Nanochitin from Marine Resources and Fabrication of Polymer Nanocomposites: Recent Advances.Polymers · 2020Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Articular cartilage degeneration is one of the most common causes of pain and disability in middle-aged and older people. Tissue engineering (TE) has shown great therapeutic promise for this condition. The design of cartilage regeneration constructs must take into account the specific characteristics of the cartilaginous matrix, as well as the avascular nature of cartilage and its cells' peculiar arrangement in isogenic groups. Keeping these factors in mind, we have designed a 3D porous scaffold based on genipin-crosslinked chitosan/chitin nanocrystals for spheroid chondral differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs) induced in hypoxic conditions. First, we demonstrated that, under low oxygen conditions, the chondrospheroids obtained express cartilage-specific markers including collagen type II (COL2A1) and aggrecan, lacking expression of osteogenic differentiation marker collagen type I (COL1A2). These results were associated with an increased expression of hypoxia-inducible factor 1α, which positively directs COL2A1 and aggrecan expression. Finally, we determined the most suitable chondrogenic differentiation pattern when hASC spheroids were seeded in the 3D porous scaffold under hypoxia and obtained a chondral extracellular matrix with a high sulphated glycosaminoglycan content, which is characteristic of articular cartilage. These findings highlight the potential use of such templates in cartilage tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.