ArticleMolecular biology and evolution2020
Evolving Populations in Biofilms Contain More Persistent Plasmids.
Article in Molecular biology and evolution, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 57 citations in OpenAlex.
- Modelling the role of the microbiome in antimicrobial resistance across scales.Nature microbiology · 2026Review
- The plasmid-host fitness landscape: a new paradigm for predicting the fate of mobile resistance.Applied and environmental microbiology · 2026Review
- HJournal of nanobiotechnology · 2025Article
- Climate warming fuels the global antibiotic resistome by altering soil bacterial traits.Nature ecology & evolution · 2025Article
- The hospital sink drain microbiome as a melting pot for AMR transmission to nosocomial pathogens.npj antimicrobials and resistance · 2025Review
- Evolution of a Plasmid Regulatory Circuit Ameliorates Plasmid Fitness Cost.Molecular biology and evolution · 2025Article
- Detection of resistance integrons among biofilm and non-biofilm producing clinical isolates ofGerms · 2024Article
- The Use of Photoactive Polymeric Nanoparticles and Nanofibers to Generate a Photodynamic-Mediated Antimicrobial Effect, with a Special Emphasis on Chronic Wounds.Pharmaceutics · 2024Review
- Plasmids, a molecular cornerstone of antimicrobial resistance in the One Health era.Nature reviews. Microbiology · 2024Review
- Biofilm tolerance, resistance and infections increasing threat of public health.Microbial cell (Graz, Austria) · 2023Review
- Growth in a biofilm promotes conjugation of amSphere · 2023Article
- Microbial Biofilms: Applications, Clinical Consequences, and Alternative Therapies.Microorganisms · 2023Review
- Preceding Host History of Conjugative Resistance Plasmids Affects Intra- and Interspecific Transfer Potential from Biofilm.mSphere · 2023Article
- Biofilms as protective cocoons against biocides: from bacterial adaptation to One Health issues.Microbiology (Reading, England) · 2023Review
- Formation and emergent dynamics of spatially organized microbial systems.Interface focus · 2023Review
- Intermittent antibiotic treatment of bacterial biofilms favors the rapid evolution of resistance.Communications biology · 2023Article
- Evolution of genome fragility enables microbial division of labor.Molecular systems biology · 2023Article
- Advances in linking single-cell bacterial stress response to population-level survival.Current opinion in biotechnology · 2023Review
- Biofilms preserve the transmissibility of a multi-drug resistance plasmid.NPJ biofilms and microbiomes · 2022Article
- Tolerance and resistance of microbial biofilms.Nature reviews. Microbiology · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
Bacterial plasmids substantially contribute to the rapid spread of antibiotic resistance, which is a crisis in healthcare today. Coevolution of plasmids and their hosts promotes this spread of resistance by ameliorating the cost of plasmid carriage. However, our knowledge of plasmid-bacteria coevolution is solely based on studies done in well-mixed liquid cultures, even though biofilms represent the main way of bacterial life on Earth and are responsible for most infections. The spatial structure and the heterogeneity provided by biofilms are known to lead to increased genetic diversity as compared with well-mixed liquids. Therefore, we expect that growth in this complex environment could affect the evolutionary trajectories of plasmid-host dyads. We experimentally evolved Shewanella oneidensis MR-1 with plasmid pBP136Gm in biofilms and chemostats and sequenced the genomes of clones and populations. Biofilm populations not only maintained a higher diversity of mutations than chemostat populations but contained a few clones with markedly more persistent plasmids that evolved via multiple distinct trajectories. These included the acquisition of a putative toxin-antitoxin transposon by the plasmid and chromosomal mutations. Some of these genetic changes resulted in loss of plasmid transferability or decrease in plasmid cost. Growth in chemostats led to a higher proportion of variants with decreased plasmid persistence, a phenomenon not detected in biofilms. We suggest that the presence of more stable plasmid-host dyads in biofilms reflects higher genetic diversity and possibly unknown selection pressures. Overall, this study underscores the importance of the mode of growth in the evolution of antibiotic-resistant bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.