Evidence map›Paper›PMID 32027361›Full record

ArticleJournal of biochemistry2020

Old but not obsolete: an enhanced high-speed immunoblot.

Sayuri L Higashi, Kazuya Yagyu, Haruna Nagase, Craig S Pearson, Herbert M Geller, Yasuhiro Katagiri

Erratum issuedOpen access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of biochemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.4field-weighted citation impact, top 34% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 1 institution in 2 countries.

Sayuri L HigashiLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Kazuya YagyuLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Haruna NagaseLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Craig S PearsonLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Herbert M GellerLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Yasuhiro KatagiriLaboratory of Developmental Neurobiology, Cell and Developmental Biology Center National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
National Institutes of Health · US

Funding

Signaling by Chondroitin Sulfate ProteoglycansZIAHL006021 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI GELLER, HERBERT M. · 2009 to 2022
$12.3M
6 · The paper itself

Abstract

The immunoblotting technique (also known as western blotting) is an essential tool used in biomedical research to determine the relative size and abundance of specific proteins and protein modifications. However, long incubation times severely limit its throughput. We have devised a system that improves antigen binding by cyclic draining and replenishing (CDR) of the antibody solution in conjunction with an immunoreaction enhancing agent. Biochemical analyses revealed that the CDR method reduced the incubation time of the antibodies, and the presence of a commercial immunoreaction enhancing agent altered the affinity of the antibody, respectively. Combination of the CDR method with the immunoreaction enhancing agent considerably enhanced the output signal and further reduced the incubation time of the antibodies. The resulting high-speed immunoblot can be completed in 20 min without any loss in sensitivity. Further, the antibodies are fully reusable. This method is effective for both chemiluminescence and fluorescence detection. Widespread adoption of this technique could dramatically boost efficiency and productivity across the life sciences.

Indexed as

AnimalsAntibodiesBiomedical EnhancementBlotting, WesternCellsCells, CulturedHumansImmunoblottingMiceMice, Inbred C57BLProteinsAntibodiesProteinschemiluminescencefluorescenceimmunoblotimmunoreaction enhancing agentwestern blot

Identifiers

PMID32027361
PMCPMC7324151
OpenAlexW3004660238

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.