Evidence map›Paper›PMID 32026660›Full record

ArticleJournal of gynecologic oncology2020

SURF4 maintains stem-like properties via BIRC3 in ovarian cancer cells.

Yongfang Yue, Lili Xia, Shanshan Xu, Conghui Wang, Xinyu Wang, Weiguo Lu, Xing Xie

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of gynecologic oncology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 16 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Yongfang Yue *Women's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0001-8453-0444
Lili Xia *Women's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0001-7821-9756
Shanshan XuWomen's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0002-1750-4171
Conghui WangWomen's Reproductive Health Laboratory of Zhejiang Province, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0001-7526-835X
Xinyu WangDepartment of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0002-3173-6390
Weiguo LuDepartment of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.ORCID https://orcid.org/0000-0002-8570-7317
Xing XieDepartment of Gynecologic Oncology, Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China. xiex@zju.edu.cn.ORCID https://orcid.org/0000-0002-1636-4816
Women's Hospital, School of Medicine, Zhejiang University · CN

Funding

Key Research and Development Program of Zhejiang Province 2019C03010National Natural Science Foundation of China 81772771
6 · The paper itself

Abstract

objectiveAs cancer stem cells (CSCs) are considered as the origin of tumor development, recurrence, and drug resistance, we aimed to explore the mechanism related to modulating stemness in CSCs, thus facilitating to search for new therapeutic strategy for ovarian cancer.

methodsIn this study, ovarian cancer stem cells (OCSCs) induced from cell line 3AO and A2780 were enriched in serum-free medium (SFM). The effect of SURF4 on CSC-like properties was evaluated by sphere-forming assays, re-differentiation assays, quantitative real-time polymerase chain reaction, flow cytometry, Western blotting, cell viability assays and in vivo xenograft experiments. The downstream molecule participating in SURF4 maintaining stemness was screened by RNA-sequencing and identified by the experiments of gene function.

resultsSURF4 was upregulated expressed in OCSCs. Knockdown of SURF4 reduced the expression of the related stem markers (SOX2 and c-MYC), inhibited self-renewal ability, and improved the sensitivity to chemotherapeutic drugs (paclitaxel and cisplatin) in OCSCs. SURF4 knockdown also inhibited tumorigenesis in nonobese diabetic/severe combined immunodeficiency mice. BIRC3 expression was controlled by SURF4, and BIRC3 showed the similar effect as SURF4 did, and BIRC3 overexpression partially recovered stem-like properties abolished by SURF4 knockdown.

conclusionOur findings suggest that SURF4 possesses the ability to maintain stemness of OCSCs via BIRC3, and may serve as a potential target in stem cell-targeted therapy for ovarian cancer.

Indexed as

Ovarian NeoplasmsAnimalsBaculoviral IAP Repeat-Containing 3 ProteinCarcinoma, Ovarian EpithelialCell Line, TumorDrug Resistance, NeoplasmFemaleHumansMembrane ProteinsMiceNeoplasm Recurrence, LocalNeoplastic Stem CellsBaculoviral IAP Repeat-Containing 3 ProteinBIRC3 protein, humanMembrane ProteinsSURF4 protein, humanSurf4 protein, mouseBIRC3Cancer Stem CellOvarian CancerSURF4

Identifiers

PMID32026660
PMCPMC7286753
OpenAlexW3004357293

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.