ArticlePhysiological reports2020
Vasoactive intestinal peptide regulates ileal goblet cell production in mice.
Article in Physiological reports, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 39 citations in OpenAlex.
- Enteric glia, neuropeptides, and Parkinson's: impacts of calcitonin gene-related peptide on gut alpha synuclein.American journal of physiology. Gastrointestinal and liver physiology · 2026Article
- Enteric nervous system-derived VIP restrains differentiation of LGR5Nature immunology · 2025Article
- Neuroepithelial VIP-VIPR1 interactions differentially control enteric type 1 and type 2 immunity.Nature immunology · 2025Article
- Development of a Caco-2-based intestinal mucosal model to study intestinal barrier properties and bacteria-mucus interactions.Gut microbes · 2025Article
- Gut neuropeptide involvement in Parkinson's disease.American journal of physiology. Gastrointestinal and liver physiology · 2025Review
- Sympathetic-Mediated Intestinal Cell Death Contributes to Gut Barrier Impairment After Stroke.Translational stroke research · 2025Article
- Akkermansia mono-colonization modulates microglia and astrocytes in a strain specific manner.Journal of neuroinflammation · 2025Article
- Apical-out intestinal organoids as an alternative model for evaluating deoxynivalenol toxicity and Lactobacillus detoxification in bovine.Scientific reports · 2024Article
- Vasoactive intestinal peptide promotes secretory differentiation and mitigates radiation-induced intestinal injury.Stem cell research & therapy · 2024Article
- Creation of a spatially complex mucus bilayer on an in vitro colon model.Scientific reports · 2024Article
- Effects of cancer-induced cachexia and administration of L-glutathione on the intestinal mucosa in rat.Amino acids · 2024Article
- Culture of vibrating microtome tissue slices as a 3D model in biomedical research.Journal of biological engineering · 2023Review
- The enteric nervous system.Physiological reviews · 2023Review
- View from the Biological Property: Insight into the Functional Diversity and Complexity of the Gut Mucus.International journal of molecular sciences · 2023Review
- Neonatal development of intestinal neuroimmune interactions.Trends in neurosciences · 2022Review
- Enteric VIP-producing neurons maintain gut microbiota homeostasis through regulating epithelium fucosylation.Cell host & microbe · 2022Article
- Efficacy of Qihuang decoction on enteric nervous system in rats after gastrectomy.Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2022Article
- The G Protein-Coupled Receptor, VPAC1, Mediates Vasoactive Intestinal Peptide-Dependent Functional Homeostasis of the Gut Microbiota.Gastro hep advances · 2022Article
- Sex differences in anatomic plasticity of gut neuronal-mast cell interactions.Physiological reports · 2021Article
- Phenolic and Non-Polar Fractions of the Extracts from Fruits, Leaves, and Twigs ofMolecules (Basel, Switzerland) · 2020Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Innervation of the intestinal mucosa has gained more attention with demonstrations of tuft and enteroendocrine cell innervation. However, the role(s) these fibers play in maintaining the epithelial and mucus barriers are still poorly understood. This study therefore examines the proximity of mouse ileal goblet cells to neuronal fibers, and the regulation of goblet cell production by vasoactive intestinal peptide (VIP). An organotypic intestinal slice model that maintains the cellular diversity of the intestinal wall ex vivo was used. An ex vivo copper-free click-reaction to label glycosaminoglycans was used to identify goblet cells. Pharmacological treatment of slices was used to assess the influence of VIP receptor antagonism on goblet cell production and neuronal fiber proximity. Goblet cells were counted and shown to have at least one peripherin immunoreactive fiber within 3 µm of the cell, 51% of the time. Treatment with a VIP receptor type I and II antagonist (VPACa) resulted in an increase in the percentage of goblet cells with peripherin fibers. Pharmacological treatments altered goblet cell counts in intestinal crypts and villi, with tetrodotoxin and VPACa substantially decreasing goblet cell counts. When cultured with 5-Ethynyl-2'-deoxyuridine (EdU) as an indicator of cell proliferation, colocalization of labeled goblet cells and EdU in ileal crypts was decreased by 77% when treated with VPACa. This study demonstrates a close relationship of intestinal goblet cells to neuronal fibers. By using organotypic slices from mouse ileum, vasoactive intestinal peptide receptor regulation of gut wall goblet cell production was revealed.
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